BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27914

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for AIMP2 121-320 double-mutant (C205S,C291S)   PubMed: 31792442

Deposition date: 2019-05-16 Original release date: 2019-09-20

Authors: Cho, Hye Young; Jeon, Young Ho; Mushtaq, Ameeq

Citation: Lim, Semi; Cho, Hye Young; Kim, Dae Gyu; Roh, Younah; Son, Se-Young; Mushtaq, Ameeq Ul; Kim, Minkyoung; Bhattarai, Deepak; Sivaraman, Aneesh; Lee, Youngjin; Lee, Jihye; Yang, Won Suk; Kim, Hoi Kyoung; Kim, Myung Hee; Lee, Kyeong; Jeon, Young Ho; Kim, Sunghoon. "Targeting the interaction of AIMP2-DX2 with HSP70 suppresses cancer development"  Nat. Chem. Biol. 16, 31-41 (2020).

Assembly members:
AIMP2 121-320 double-mutant (C205S,C291S), polymer, 200 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
AIMP2 121-320 double-mutant (C205S,C291S): DIVINANPASPPLSLLVLHR LLCEHFRVLSTVHTHSSVKS VPENLLKCFGEQNKKQPRQD YQLGFTLIWKNVPKTQMKFS IQTMSPIEGEGNIARFLFSL FGQKHNAVNATLIDSWVDIA IFQLKEGSSKEKAAVFRSMN SALGKSPWLAGNELTVADVV LWSVLQQIGGSSVTVPANVQ RWMRSCENLAPFNTALKLLK

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts190
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AIMP2 121-320 double-mutant (C205S,C291S)1

Entities:

Entity 1, AIMP2 121-320 double-mutant (C205S,C291S) 200 residues - Formula weight is not available

1   ASPILEVALILEASNALAASNPROALASER
2   PROPROLEUSERLEULEUVALLEUHISARG
3   LEULEUCYSGLUHISPHEARGVALLEUSER
4   THRVALHISTHRHISSERSERVALLYSSER
5   VALPROGLUASNLEULEULYSCYSPHEGLY
6   GLUGLNASNLYSLYSGLNPROARGGLNASP
7   TYRGLNLEUGLYPHETHRLEUILETRPLYS
8   ASNVALPROLYSTHRGLNMETLYSPHESER
9   ILEGLNTHRMETSERPROILEGLUGLYGLU
10   GLYASNILEALAARGPHELEUPHESERLEU
11   PHEGLYGLNLYSHISASNALAVALASNALA
12   THRLEUILEASPSERTRPVALASPILEALA
13   ILEPHEGLNLEULYSGLUGLYSERSERLYS
14   GLULYSALAALAVALPHEARGSERMETASN
15   SERALALEUGLYLYSSERPROTRPLEUALA
16   GLYASNGLULEUTHRVALALAASPVALVAL
17   LEUTRPSERVALLEUGLNGLNILEGLYGLY
18   SERSERVALTHRVALPROALAASNVALGLN
19   ARGTRPMETARGSERCYSGLUASNLEUALA
20   PROPHEASNTHRALALEULYSLEULEULYS

Samples:

sample_1: AIMP2 121-320 double-mutant (C205S,C291S)' '[U-99% 13C; U-99% 15N]; natural abundance; natural abundance; natural abundance; natural abundance; natural abundance; natural abundance

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts