BMRB Entry 27830

Title:
Backbone 1H, 13C and 15N Chemical Shift Assignment of complex of MarH with L-Trp
Deposition date:
2019-03-07
Original release date:
2019-04-11
Authors:
Liu, Bin; Ma, Xiaofang; Hu, Kaifeng
Citation:

Citation: Liu, Bin; Hou, Yan; Wang, Xiaozheng; Ma, Xiaofang; Fang, Shiqi; Huang, Tao; Chen, Yanli; Bai, Zhiqiang; Lin, Shuangjun; Zhang, Rundong; Hu, Kaifeng. "Structural basis of the mechanism of beta-methyl epimerization by enzyme MarH"  Org. Biomol. Chem. 17, 9605-9614 (2019).
PubMed: 31681917

Assembly members:

Assembly members:
MarH, polymer, 126 residues, Formula weight is not available
entity_TRP, non-polymer, 204.225 Da.

Natural source:

Natural source:   Common Name: Streptomyces   Taxonomy ID: 1883   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts103
1H chemical shifts484

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MarH1
2L-Tryptophan2

Entities:

Entity 1, MarH 126 residues - Formula weight is not available

1   GLYSERARGPROALAASPPROGLUILEVAL
2   GLUGLYLEUPROILEPROLEUALAVALALA
3   GLYHISHISGLNPROALAPROPHETYRLEU
4   THRALAASPMETPHEGLYGLYLEUPROVAL
5   GLNLEUALAGLYGLYGLULEUSERTHRLEU
6   VALGLYLYSPROVALALAALAPROHISTHR
7   HISPROVALASPGLULEUTYRLEULEUVAL
8   SERPROASNLYSGLYGLYALAARGILEGLU
9   VALGLNLEUASPGLYARGARGHISGLULEU
10   LEUSERPROALAVALMETARGILEPROALA
11   GLYSERGLUHISCYSPHELEUTHRLEUGLU
12   ALAGLUVALGLYSERTYRCYSPHEGLYILE
13   LEULEUGLYASPARGLEU

Entity 2, L-Tryptophan - C11 H12 N2 O2 - 204.225 Da.

1   TRP

Samples:

sample_1: MarH, [U-100% 13C; U-100% 15N], 0.65 mM; L-Tryptophan 2 mM; sodium phosphate 50 mM; potassium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

AutoAssign, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks