BMRB Entry 27795

Title:
FLNa4-6
Deposition date:
2019-02-21
Original release date:
2020-02-28
Authors:
Haataja, Tatu; Permi, Perttu
Citation:

Citation: Haataja, Tatu; Capoulade, Romain; Lecointe, Simon; Hellman, Maarit; Merot, Jean; Permi, Perttu; Pentikainen, Ulla. "Critical Structural Defects Explain Filamin A Mutations Causing Mitral Valve Dysplasia"  Biophys. J. 117, 1467-1475 (2019).
PubMed: 31542223

Assembly members:

Assembly members:
FLNa4-6, polymer, 298 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGTvL1-SGC

Data sets:
Data typeCount
13C chemical shifts556
15N chemical shifts245
1H chemical shifts305

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FLNa4-61

Entities:

Entity 1, FLNa4-6 298 residues - Formula weight is not available

1   SERMETCYSGLYASNGLNLYSVALARGALA
2   TRPGLYPROGLYLEUGLUGLYGLYVALVAL
3   GLYLYSSERALAASPPHEVALVALGLUALA
4   ILEGLYASPASPVALGLYTHRLEUGLYPHE
5   SERVALGLUGLYPROSERGLNALALYSILE
6   GLUCYSASPASPLYSGLYASPGLYSERCYS
7   ASPVALARGTYRTRPPROGLNGLUALAGLY
8   GLUTYRALAVALHISVALLEUCYSASNSER
9   GLUASPILEARGLEUSERPROPHEMETALA
10   ASPILEARGASPALAPROGLNASPPHEHIS
11   PROASPARGVALLYSALAARGGLYPROGLY
12   LEUGLULYSTHRGLYVALALAVALASNLYS
13   PROALAGLUPHETHRVALASPALALYSHIS
14   GLYGLYLYSALAPROLEUARGVALGLNVAL
15   GLNASPASNGLUGLYCYSPROVALGLUALA
16   LEUVALLYSASPASNGLYASNGLYTHRTYR
17   SERCYSSERTYRVALPROARGLYSPROVAL
18   LYSHISTHRALAMETVALSERTRPGLYGLY
19   VALSERILEPROASNSERPROPHEARGVAL
20   ASNVALGLYALAGLYSERHISPROASNLYS
21   VALLYSVALTYRGLYPROGLYVALALALYS
22   THRGLYLEULYSALAHISGLUPROTHRTYR
23   PHETHRVALASPCYSALAGLUALAGLYGLN
24   GLYASPVALSERILEGLYILELYSCYSALA
25   PROGLYVALVALGLYPROALAGLUALAASP
26   ILEASPPHEASPILEILEARGASNASPASN
27   ASPTHRPHETHRVALLYSTYRTHRPROARG
28   GLYALAGLYSERTYRTHRILEMETVALLEU
29   PHEALAASPGLNALATHRPROTHRSERPRO
30   ILEARGVALLYSVALGLUPROSER

Samples:

sample_1: FLNa4-6, [U-100% 13C; U-100% 15N], 0.8 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P21333
AlphaFold Q8NF52

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks