BMRB Entry 27747

Title:
Assignment of the ARC4 domain of human Tankyrase
Deposition date:
2019-01-07
Original release date:
2019-04-12
Authors:
Pfuhl, Mark; Guettler, Sebastian; Zaleska, Mariola
Citation:

Citation: Zaleska, Mariola; Pollock, Katie; Collins, Ian; Guettler, Sebastian; Pfuhl, Mark. "Assignment of the ARC4 domain of human Tankyrase"  Biomol. NMR Assign. 13, 255-260 (2019).
PubMed: 30847846

Assembly members:

Assembly members:
ARC4, polymer, 165 residues, 17830 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM30

Data sets:
Data typeCount
15N chemical shifts174
1H chemical shifts186

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ARC4 domain of human Tankyrase1

Entities:

Entity 1, ARC4 domain of human Tankyrase 165 residues - 17830 Da.

the first three residues are a cloning artifact

1   GLYALAMETGLYASNSERGLUALAASPARG
2   GLNLEULEUGLUALAALALYSALAGLYASP
3   VALGLUTHRVALLYSLYSLEUCYSTHRVAL
4   GLNSERVALASNCYSARGASPILEGLUGLY
5   ARGGLNSERTHRPROLEUHISPHEALAALA
6   GLYTYRASNARGVALSERVALVALGLUTYR
7   LEULEUGLNHISGLYALAASPVALHISALA
8   LYSASPLYSGLYGLYLEUVALPROLEUHIS
9   ASNALACYSSERTYRGLYHISTYRGLUVAL
10   ALAGLULEULEUVALLYSHISGLYALAVAL
11   VALASNVALALAASPLEUTRPLYSPHETHR
12   PROLEUHISGLUALAALAALALYSGLYLYS
13   TYRGLUILECYSLYSLEULEULEUGLNHIS
14   GLYALAASPPROTHRLYSLYSASNARGASP
15   GLYASNTHRPROLEUASPLEUVALLYSASP
16   GLYASPTHRASPILEGLNASPLEULEUARG
17   GLYASPALAALALEU

Samples:

sample_1: ARC4, [U-98% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: ARC4, [U-95% 13C; U-95% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D (H)C(CCO)NHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

CCPnmr v2.4, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks