BMRB Entry 27729
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27729
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Eisenmesser, Elan; Gottschlich, Adrian; Redzic, Jasmina; Paukovich, Natasia; Nix, Jay; Azam, Tania; Zhang, Lingdi; Zhao, Rui; Kieft, Jeffrey; The, Erlinda; Meng, Xianzhong; Dinarello, Charles. "Interleukin-37 monomer is the active form for reducing innate immunity." Proc. Natl. Acad. Sci. U.S.A. 116, 5514-5522 (2019).
PubMed: 30819901
Assembly members:
IL37_dimer, polymer, 218 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 444 |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 249 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Il37 chain A | 1 |
| 2 | Il37 chain B | 1 |
Entities:
Entity 1, Il37 chain A 218 residues - Formula weight is not available
| 1 | MET | SER | PHE | VAL | GLY | GLU | ASN | SER | GLY | VAL | ||||
| 2 | LYS | MET | GLY | SER | GLU | ASP | TRP | GLU | LYS | ASP | ||||
| 3 | GLU | PRO | GLN | CYS | CYS | LEU | GLU | ASP | PRO | ALA | ||||
| 4 | GLY | SER | PRO | LEU | GLU | PRO | GLY | PRO | SER | LEU | ||||
| 5 | PRO | THR | MET | ASN | PHE | VAL | HIS | THR | SER | PRO | ||||
| 6 | LYS | VAL | LYS | ASN | LEU | ASN | PRO | LYS | LYS | PHE | ||||
| 7 | SER | ILE | HIS | ASP | GLN | ASP | HIS | LYS | VAL | LEU | ||||
| 8 | VAL | LEU | ASP | SER | GLY | ASN | LEU | ILE | ALA | VAL | ||||
| 9 | PRO | ASP | LYS | ASN | TYR | ILE | ARG | PRO | GLU | ILE | ||||
| 10 | PHE | PHE | ALA | LEU | ALA | SER | SER | LEU | SER | SER | ||||
| 11 | ALA | SER | ALA | GLU | LYS | GLY | SER | PRO | ILE | LEU | ||||
| 12 | LEU | GLY | VAL | SER | LYS | GLY | GLU | PHE | CYS | LEU | ||||
| 13 | TYR | CYS | ASP | LYS | ASP | LYS | GLY | GLN | SER | HIS | ||||
| 14 | PRO | SER | LEU | GLN | LEU | LYS | LYS | GLU | LYS | LEU | ||||
| 15 | MET | LYS | LEU | ALA | ALA | GLN | LYS | GLU | SER | ALA | ||||
| 16 | ARG | ARG | PRO | PHE | ILE | PHE | TYR | ARG | ALA | GLN | ||||
| 17 | VAL | GLY | SER | TRP | ASN | MET | LEU | GLU | SER | ALA | ||||
| 18 | ALA | HIS | PRO | GLY | TRP | PHE | ILE | CYS | THR | SER | ||||
| 19 | CYS | ASN | CYS | ASN | GLU | PRO | VAL | GLY | VAL | THR | ||||
| 20 | ASP | LYS | PHE | GLU | ASN | ARG | LYS | HIS | ILE | GLU | ||||
| 21 | PHE | SER | PHE | GLN | PRO | VAL | CYS | LYS | ALA | GLU | ||||
| 22 | MET | SER | PRO | SER | GLU | VAL | SER | ASP |
Samples:
sample_1: IL37 dimer, [U-100% 15N], 1 mM; Phosphate 50 mM; NaCl 150 mM; EDTA 1 mM
sample_2: IL37 dimer, [U-13C; U-15N; U-2H], 1 mM; Phosphate 50 mM; NaCl 150 mM; EDTA 1 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_2 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks