BMRB Entry 27692

Title:
Backbone Chemical Shift Assignments for Ca+-Bound Calmodulin Mutant (D21A/D23A/D25A/E32A D57A/D59A/N61A/E68A) Bound to CaV1.2 IQ Motif
Deposition date:
2018-11-16
Original release date:
2019-03-28
Authors:
Ames, James; Salveson, Ian
Citation:

Citation: Salveson, Ian; Anderson, David; Hell, Johannes; Ames, James. "Chemical shift assignments of a calmodulin intermediate with two Ca2+ bound in complex with the IQ-motif of voltage-gated Ca2+ channels (CaV1.2)"  Biomol. NMR Assign. 13, 233-237 (2019).
PubMed: 30788773

Assembly members:

Assembly members:
Calmodulin, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 11

Data sets:
Data typeCount
13C chemical shifts442
15N chemical shifts124
1H chemical shifts538

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin1

Entities:

Entity 1, Calmodulin 149 residues - Formula weight is not available

1   METALAASPGLNLEUTHRGLUGLUGLNILE
2   ALAGLUPHELYSGLUALAPHESERLEUPHE
3   ALALYSALAGLYALAGLYTHRILETHRTHR
4   LYSALALEUGLYTHRVALMETARGSERLEU
5   GLYGLNASNPROTHRGLUALAGLULEUGLN
6   ASPMETILEASNGLUVALALAALAALAGLY
7   ALAGLYTHRILEASPPHEPROALAPHELEU
8   THRMETMETALAARGLYSMETLYSASPTHR
9   ASPSERGLUGLUGLUILEARGGLUALAPHE
10   ARGVALPHEASPLYSASPGLYASNGLYTYR
11   ILESERALAALAGLULEUARGHISVALMET
12   THRASNLEUGLYGLULYSLEUTHRASPGLU
13   GLUVALASPGLUMETILEARGGLUALAASP
14   ILEASPGLYASPGLYGLNVALASNTYRGLU
15   GLUPHEVALGLNMETMETTHRALALYS

Samples:

sample_1: Calmodulin, [U-100% 13C; U-100% 15N; U-80% 2H], 0.75 mM; CaV1.2 IQ motif 1 mM; CaCl2 2mM mM

sample_2: Calmodulin, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; CaV1.2 IQ motif 0.75 mM; CaCl2 2 mM

sample_conditions_1: ionic strength: 0.2338 M; pH: 7.5; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 0.15992 M; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks