BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27689

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Vibrio cholerae DciA 1-111

Deposition date: 2018-11-12 Original release date: 2021-05-19

Authors: Andreani, Jessica; Ochsenbein, Francoise; Quevillon-Cheruel, Sophie

Citation: Marsin, Stephanie; Andreani, Jessica; Baconnais, Sonia; Gallay-Li de la Sierra, Ines; Legrand, Pierre; Durand, Dominique; Adam, Yazid; Aumont, Magali; Velours, Christophe; Ochsenbein, Francoise; Le Cam, Eric; Quevillon-Cheruel, Sophie; Ferat, Jean-Luc. "DciA is a helicase mediator that loads and activates bacterial replicative helicases"  Nucleic Acids Res. ., .-..

Assembly members:
DciA, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: V. cholerae   Taxonomy ID: 666   Superkingdom: Bacteria   Kingdom: Eubacteria   Genus/species: Vibrio cholerae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):
DciA: MHHHHHHRDHRPTATDELIQ ASKLKQIQEHAKAILLINRQ LQDILPKGLKTQVRAANVRG GNLVLEAASAALKMKVDYER LHILTQLRQNGFGHLISIEV RVNPELYRQSKITSEDA

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts107
1H chemical shifts368

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DciA monomer1

Entities:

Entity 1, DciA monomer 117 residues - Formula weight is not available

1   METHISHISHISHISHISHISARGASPHIS
2   ARGPROTHRALATHRASPGLULEUILEGLN
3   ALASERLYSLEULYSGLNILEGLNGLUHIS
4   ALALYSALAILELEULEUILEASNARGGLN
5   LEUGLNASPILELEUPROLYSGLYLEULYS
6   THRGLNVALARGALAALAASNVALARGGLY
7   GLYASNLEUVALLEUGLUALAALASERALA
8   ALALEULYSMETLYSVALASPTYRGLUARG
9   LEUHISILELEUTHRGLNLEUARGGLNASN
10   GLYPHEGLYHISLEUILESERILEGLUVAL
11   ARGVALASNPROGLULEUTYRARGGLNSER
12   LYSILETHRSERGLUASPALA

Samples:

sample_1: DciA, [U-100% 13C; U-100% 15N], 0.9 mM; DSS 0.2 mM; sodium azide 0.1 mg/mL; EDTA 2 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N SOFAST HMQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts