BMRB Entry 27614

Title:
NMR resonance assignments of the TPR domain of human AIPL1
Deposition date:
2018-09-16
Original release date:
2019-09-17
Authors:
Yu, Liping; Yadav, Ravi; Artemyev, Nikolai
Citation:

Citation: Yu, Liping; Yadav, Ravi; Artemyev, Nikolai. "NMR resonance assignments of the TPR domain of human aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1)"  Biomol. NMR Assign. 13, 79-83 (2019).
PubMed: 30341566

Assembly members:

Assembly members:
AIPL1-TPR, polymer, 155 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts404
15N chemical shifts129
1H chemical shifts257

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AIPL1-TPR1

Entities:

Entity 1, AIPL1-TPR 155 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISGLYASN
2   HISGLULYSMETLYSALAVALPROVALLEU
3   HISGLYGLUGLYASNARGLEUPHELYSLEU
4   GLYARGTYRGLUGLUALASERSERLYSTYR
5   GLNGLUALAILEILECYSLEUARGASNLEU
6   GLNTHRLYSGLULYSPROTRPGLUVALGLN
7   TRPLEULYSLEUGLULYSMETILEASNTHR
8   LEUILELEUASNTYRCYSGLNCYSLEULEU
9   LYSLYSGLUGLUTYRTYRGLUVALLEUGLU
10   HISTHRSERASPILELEUARGHISHISPRO
11   GLYILEVALLYSALATYRTYRVALARGALA
12   ARGALAHISALAGLUVALTRPASNGLUALA
13   GLUALALYSALAASPLEUGLNLYSVALLEU
14   GLULEUGLUPROSERMETGLNLYSALAVAL
15   ARGARGGLULEUARGLEULEUGLUASNARG
16   METALAGLULYSGLN

Samples:

sample_1: AIPL1-TPR, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 700 mM; DTT 6.0 mM

sample_conditions_1: ionic strength: 1.62 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks