BMRB Entry 27610

Title:
Sequence-specific 1H, 13C, and 15N backbone assignment of Fibronectin type III 14
Deposition date:
2018-09-13
Original release date:
2018-10-01
Authors:
Zhong, Xueyin; Arnolds, Oliver; Krenczyk, Oktavian; Gajewski, Jana; Puetz, Stefanie; Herrmann, Christian; Stoll, Raphael
Citation:

Citation: Zhong, Xueyin; Arnolds, Oliver; Krenczyk, Oktavian; Gajewski, Jana; Puetz, Stefanie; Herrmann, Christian; Stoll, Raphael. "Structure in solution of fibronectin type III-domain 14 reveals its synergistic heparin binding site"  Biochemistry 57, 6045-6049 (2018).
PubMed: 30260627

Assembly members:

Assembly members:
FNIII14, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-19

Data sets:
Data typeCount
13C chemical shifts389
15N chemical shifts80
1H chemical shifts624

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FNIII141

Entities:

Entity 1, FNIII14 91 residues - Formula weight is not available

1   GLYHISMETASPALAPROSERASNLEUARG
2   PHELEUALATHRTHRPROASNSERLEULEU
3   VALSERTRPGLNPROPROARGALAARGILE
4   THRGLYTYRILEILELYSTYRGLULYSPRO
5   GLYSERPROPROARGGLUVALVALPROARG
6   PROARGPROGLYVALTHRGLUALATHRILE
7   THRGLYLEUGLUPROGLYTHRGLUTYRTHR
8   ILETYRVALILEALALEULYSASNASNGLN
9   LYSSERGLUPROLEUILEGLYARGLYSLYS
10   THR

Samples:

sample_1: FNIII14, [U-13C; U-15N], 1 mM; NaCl 137 mM; KCl 2.7 mM; Na2HPO4 10 mM; KH2PO4 1.4 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 0.163 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR, Bruker Biospin, CCPN - chemical shift assignment, processing

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks