BMRB Entry 27573

Title:
Backbone and side chain chemical shifts assignments of uniformly labeled 13C, 15N SUMO1-F64L
Deposition date:
2018-08-08
Original release date:
2019-03-01
Authors:
Das, Ranabir; Chatterjee, Kiran Sankar
Citation:

Citation: Chatterjee, Kiran Sankar; Tripathi, Vasvi; Das, Ranabir. "A conserved and buried edge-to-face aromatic interaction in small ubiquitin-like modifier (SUMO) has a role in SUMO stability and function"  J. Biol. Chem. 294, 6772-6784 (2019).
PubMed: 30824543

Assembly members:

Assembly members:
SUMO1_F64L, polymer, 109 residues, 12497 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-80L

Data sets:
Data typeCount
13C chemical shifts197
15N chemical shifts86
1H chemical shifts472

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SUMO1 F64L1

Entities:

Entity 1, SUMO1 F64L 109 residues - 12497 Da.

residue 1 to 12 is His affinity tag

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERMETSERASPGLNGLUALALYSPRO
3   SERTHRGLUASPLEUGLYASPLYSLYSGLU
4   GLYGLUTYRILELYSLEULYSVALILEGLY
5   GLNASPSERSERGLUILEHISPHELYSVAL
6   LYSMETTHRTHRHISLEULYSLYSLEULYS
7   GLUSERTYRCYSGLNARGGLNGLYVALPRO
8   METASNSERLEUARGLEULEUPHEGLUGLY
9   GLNARGILEALAASPASNHISTHRPROLYS
10   GLULEUGLYMETGLUGLUGLUASPVALILE
11   GLUVALTYRGLNGLUGLNTHRGLYGLY

Samples:

sample_1: SUMO1 F64L, [U-100% 13C; U-100% 15N], 225 uM; DTT 2 mM; Phosphate buffer 25 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

NMRDraw, Bahrami, Markley, Assadi, and Eghbalnia, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks