BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27555

Title: Backbone assignment of Rip2Card   PubMed: 30279485

Deposition date: 2018-07-24 Original release date: 2018-11-15

Authors: Wallmann, Arndt; Oschkinat, Hartmut

Citation: Pellegrini, Erika; Desfosses, Ambroise; Wallmann, Arndt; Schulze, Wiebke; Rehbein, Kristina; Mas, Philippe; Signor, Luca; Gaudon, Stephanie; Zenkeviciute, Grasilda; Malet, Helene; Gutsche, Irina; Sachse, Carsten; Schoehn, Guy; Oschkinat, Hartmut; Cusack, Stephen. "RIP2 filament formation is required for NOD2 dependent NF-B signalling"  Nat. Commun. 9, 4043-4043 (2018).

Assembly members:
Rip2Card, polymer, 107 residues, 12248.0952 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM40

Entity Sequences (FASTA):
Rip2Card: AIAQQWIQSKREDIVNQMTE ACLNQSLDALLSRDLIMKED YELVSTKPTRTSKVRQLLDT TDIQGEEFAKVIVQKLKDNK QMGLQPYPEILVVSRSPSLN LLQNKSM

Data sets:
Data typeCount
13C chemical shifts185
15N chemical shifts63
1H chemical shifts63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CARD domain1

Entities:

Entity 1, CARD domain 107 residues - 12248.0952 Da.

1   ALAILEALAGLNGLNTRPILEGLNSERLYS
2   ARGGLUASPILEVALASNGLNMETTHRGLU
3   ALACYSLEUASNGLNSERLEUASPALALEU
4   LEUSERARGASPLEUILEMETLYSGLUASP
5   TYRGLULEUVALSERTHRLYSPROTHRARG
6   THRSERLYSVALARGGLNLEULEUASPTHR
7   THRASPILEGLNGLYGLUGLUPHEALALYS
8   VALILEVALGLNLYSLEULYSASPASNLYS
9   GLNMETGLYLEUGLNPROTYRPROGLUILE
10   LEUVALVALSERARGSERPROSERLEUASN
11   LEULEUGLNASNLYSSERMET

Samples:

sample_1: Rip2Card, [U-100% 13C; U-100% 15N; 100% D2O], 2 mg

sample_conditions_1: pH: 8; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D (H)CANHsample_1isotropicsample_conditions_1
3D (HCO)CA(CO)NHsample_1isotropicsample_conditions_1
3D (HCA)CB(CA)NHsample_1isotropicsample_conditions_1
3D (HCA)CB(CACO)NHsample_1isotropicsample_conditions_1
3D (H)CONHsample_1isotropicsample_conditions_1
3D (H)CO(CA)NHsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, collection, processing

Bruker Biospin, Bruker Biospin - chemical shift assignment, collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts