BMRB Entry 27496

Title:
PLCg1 constructs
Deposition date:
2018-05-29
Original release date:
2018-07-02
Authors:
Koss, Hans; Driscoll, Paul
Citation:

Citation: Koss, Hans; Bunney, Tom; Esposito, Diego; Martins, Marta; Katan, Matilda; Driscoll, Paul. "Dynamic allostery in PLC 1 and its modulation by a cancer mutation revealed by MD simulation and NMR"  Biophys. J. 115, 31-45 (2018).
PubMed: 29972810

Assembly members:

Assembly members:
phospholipase_Cg1, polymer, 246 residues, Formula weight is not available
pY783_peptide, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOPINS

Data typeCount
13C chemical shifts856
15N chemical shifts813
1H chemical shifts813

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PLCg1 tandem SH21
2NSH2CSH2DCTPY7832

Entities:

Entity 1, PLCg1 tandem SH2 246 residues - Formula weight is not available

1   HISSERASNGLULYSTRPPHEHISGLYLYS
2   LEUGLYALAGLYARGASPGLYARGHISILE
3   ALAGLUARGLEULEUTHRGLUTYRCYSILE
4   GLUTHRGLYALAPROASPGLYSERPHELEU
5   VALARGGLUSERGLUTHRPHEVALGLYASP
6   TYRTHRLEUSERPHETRPARGASNGLYLYS
7   VALGLNHISCYSARGILEHISSERARGGLN
8   ASPALAGLYTHRPROLYSPHEPHELEUTHR
9   ASPASNLEUVALPHEASPSERLEUTYRASP
10   LEUILETHRHISTYRGLNGLNVALPROLEU
11   ARGCYSASNGLUPHEGLUMETARGLEUSER
12   GLUPROVALPROGLNTHRASNALAHISGLU
13   SERLYSGLUTRPTYRHISALASERLEUTHR
14   ARGALAGLNALAGLUHISMETLEUMETARG
15   VALPROARGASPGLYALAPHELEUVALARG
16   LYSARGASNGLUPROASNSERTYRALAILE
17   SERPHEARGALAGLUGLYLYSILELYSHIS
18   CYSARGVALGLNGLNGLUGLYGLNTHRVAL
19   METLEUGLYASNSERGLUPHEASPSERLEU
20   VALASPLEUILESERTYRTYRGLULYSHIS
21   PROLEUTYRARGLYSMETLYSLEUARGTYR
22   PROILEASNGLUGLUALALEUGLULYSILE
23   GLYTHRALAGLUPROASPPHEGLYALALEU
24   PHEGLUGLYARGASNPROGLYPHETYRVAL
25   GLUALAASNPROMETPRO

Entity 2, NSH2CSH2DCTPY783 13 residues - Formula weight is not available

1   ASNPROGLYPHEPROTYRVALGLUALAASN
2   PROMETPRO

Samples:

sample_1: phospholipase Cg1, [U-13C; U-15N], 200 mM; phospholipase Cg1, [U-13C; U-15N; U-2H], 175 uM; phospholipase Cg1, [U-13C; U-15N], 175 uM; Na2HPO4/NaH2PO4 25 mM; NaCl 50 mM; DTT 5 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.075 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CCPN_Analysis, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks