BMRB Entry 27445

Name: Backbone Assignment of GII.4 Saga Norovirus Protruding Domain
Published: 2018-09-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27445

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignment of GII.4 Saga Norovirus Protruding Domain

Deposition date:

2018-04-10

Original release date:

2018-09-18

Authors:

Creutznacher, Robert; Mallagaray, Alvaro; Grimm, Lena; Peters, Thomas

Citation:

Citation: Bucher, Katharina Susanne; Yan, Hao; Creutznacher, Robert; Ruoff, Kerstin; Mallagaray, Alvaro; Grafmuller, Andrea; Dirks, Jan Sebastian; Kilic, Turgay; Weickert, Sabrina; Rubailo, Anna; Drescher, Malte; Schmidt, Stephan; Hansman, Grant; Peters, Thomas; Uetrecht, Charlotte; Hartmann, Laura. "Fucose-Functionalized Precision Glycomacromolecules Targeting Human Norovirus Capsid Protein." Biomacromolecules 19, 3714-3724 (2018).
PubMed: 30071731

Assembly members:

Assembly members:
GII.4 Saga Norovirus Protruding Domain, polymer, 310 residues, 34068.2246 Da.

Natural source:

Natural source: Common Name: Norovirus Taxonomy ID: 142786 Superkingdom: Viruses Kingdom: not available Genus/species: Norovirus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: -

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GII.4 Saga Norovirus Protruding Domain: GPGSKPFTVPILTVEEMTNS RFPIPLEKLFTGPSGAFVVQ PQNGRCTTDGVLLGTTQLSP VNICTFRGDVTHIAGSRNYT MNLASLNWNNYDPTEEIPAP LGTPDFVGKIQGLLTQTTKG DGSTRGHKATVYTGSAPFTP KLGSVQFSTDTENDFETHQN TKFTPVGVIQDGSTTHRNEP QQWVLPSYSGRNVHNVHLAP AVAPTFPGEQLLFFRSTMPG CSGYPNMDLDCLLPQEWVQH FYQEAAPAQSDVALLRFVNP DTGRVLFECKLHKSGYVTVA HTGQHDLVIPPNGYFRFDSW VNQFYTLAPM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	767
15N chemical shifts	249
1H chemical shifts	249

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GII.4 Saga Norovirus Protruding Domain, chain 1	1
2	GII.4 Saga Norovirus Protruding Domain, chain 2	1

Entities:

Entity 1, GII.4 Saga Norovirus Protruding Domain, chain 1 310 residues - 34068.2246 Da.

1	GLY	PRO	GLY	SER	LYS	PRO	PHE	THR	VAL	PRO
2	ILE	LEU	THR	VAL	GLU	GLU	MET	THR	ASN	SER
3	ARG	PHE	PRO	ILE	PRO	LEU	GLU	LYS	LEU	PHE
4	THR	GLY	PRO	SER	GLY	ALA	PHE	VAL	VAL	GLN
5	PRO	GLN	ASN	GLY	ARG	CYS	THR	THR	ASP	GLY
6	VAL	LEU	LEU	GLY	THR	THR	GLN	LEU	SER	PRO
7	VAL	ASN	ILE	CYS	THR	PHE	ARG	GLY	ASP	VAL
8	THR	HIS	ILE	ALA	GLY	SER	ARG	ASN	TYR	THR
9	MET	ASN	LEU	ALA	SER	LEU	ASN	TRP	ASN	ASN
10	TYR	ASP	PRO	THR	GLU	GLU	ILE	PRO	ALA	PRO
11	LEU	GLY	THR	PRO	ASP	PHE	VAL	GLY	LYS	ILE
12	GLN	GLY	LEU	LEU	THR	GLN	THR	THR	LYS	GLY
13	ASP	GLY	SER	THR	ARG	GLY	HIS	LYS	ALA	THR
14	VAL	TYR	THR	GLY	SER	ALA	PRO	PHE	THR	PRO
15	LYS	LEU	GLY	SER	VAL	GLN	PHE	SER	THR	ASP
16	THR	GLU	ASN	ASP	PHE	GLU	THR	HIS	GLN	ASN
17	THR	LYS	PHE	THR	PRO	VAL	GLY	VAL	ILE	GLN
18	ASP	GLY	SER	THR	THR	HIS	ARG	ASN	GLU	PRO
19	GLN	GLN	TRP	VAL	LEU	PRO	SER	TYR	SER	GLY
20	ARG	ASN	VAL	HIS	ASN	VAL	HIS	LEU	ALA	PRO
21	ALA	VAL	ALA	PRO	THR	PHE	PRO	GLY	GLU	GLN
22	LEU	LEU	PHE	PHE	ARG	SER	THR	MET	PRO	GLY
23	CYS	SER	GLY	TYR	PRO	ASN	MET	ASP	LEU	ASP
24	CYS	LEU	LEU	PRO	GLN	GLU	TRP	VAL	GLN	HIS
25	PHE	TYR	GLN	GLU	ALA	ALA	PRO	ALA	GLN	SER
26	ASP	VAL	ALA	LEU	LEU	ARG	PHE	VAL	ASN	PRO
27	ASP	THR	GLY	ARG	VAL	LEU	PHE	GLU	CYS	LYS
28	LEU	HIS	LYS	SER	GLY	TYR	VAL	THR	VAL	ALA
29	HIS	THR	GLY	GLN	HIS	ASP	LEU	VAL	ILE	PRO
30	PRO	ASN	GLY	TYR	PHE	ARG	PHE	ASP	SER	TRP
31	VAL	ASN	GLN	PHE	TYR	THR	LEU	ALA	PRO	MET

Samples:

Sample_1: Molecule 1, [U-13C; U-15N; U-2H], 0.15 mM; sodium phosphate buffer 20 mM

CondSet1: pH: 7.300; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HN(CO)CACB	Sample_1	isotropic	CondSet1
3D HNCACB	Sample_1	isotropic	CondSet1
3D HNCO	Sample_1	isotropic	CondSet1
3D HN(CO)CA	Sample_1	isotropic	CondSet1
3D HN(CA)CO	Sample_1	isotropic	CondSet1
3D HNCO	Sample_1	isotropic	CondSet1
3D HNCACB	Sample_1	isotropic	CondSet1
3D HNCA	Sample_1	isotropic	CondSet1
3D HN(CO)CA	Sample_1	isotropic	CondSet1

Software:

CcpNmr_Analysis v2.4, CCPN - Assignment

TopSpin v3.5, Bruker - Data Processing

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 900 MHz

1	GLY	PRO	GLY	SER	LYS	PRO	PHE	THR	VAL	PRO
2	ILE	LEU	THR	VAL	GLU	GLU	MET	THR	ASN	SER
3	ARG	PHE	PRO	ILE	PRO	LEU	GLU	LYS	LEU	PHE
4	THR	GLY	PRO	SER	GLY	ALA	PHE	VAL	VAL	GLN
5	PRO	GLN	ASN	GLY	ARG	CYS	THR	THR	ASP	GLY
6	VAL	LEU	LEU	GLY	THR	THR	GLN	LEU	SER	PRO
7	VAL	ASN	ILE	CYS	THR	PHE	ARG	GLY	ASP	VAL
8	THR	HIS	ILE	ALA	GLY	SER	ARG	ASN	TYR	THR
9	MET	ASN	LEU	ALA	SER	LEU	ASN	TRP	ASN	ASN
10	TYR	ASP	PRO	THR	GLU	GLU	ILE	PRO	ALA	PRO
11	LEU	GLY	THR	PRO	ASP	PHE	VAL	GLY	LYS	ILE
12	GLN	GLY	LEU	LEU	THR	GLN	THR	THR	LYS	GLY
13	ASP	GLY	SER	THR	ARG	GLY	HIS	LYS	ALA	THR
14	VAL	TYR	THR	GLY	SER	ALA	PRO	PHE	THR	PRO
15	LYS	LEU	GLY	SER	VAL	GLN	PHE	SER	THR	ASP
16	THR	GLU	ASN	ASP	PHE	GLU	THR	HIS	GLN	ASN
17	THR	LYS	PHE	THR	PRO	VAL	GLY	VAL	ILE	GLN
18	ASP	GLY	SER	THR	THR	HIS	ARG	ASN	GLU	PRO
19	GLN	GLN	TRP	VAL	LEU	PRO	SER	TYR	SER	GLY
20	ARG	ASN	VAL	HIS	ASN	VAL	HIS	LEU	ALA	PRO
21	ALA	VAL	ALA	PRO	THR	PHE	PRO	GLY	GLU	GLN
22	LEU	LEU	PHE	PHE	ARG	SER	THR	MET	PRO	GLY
23	CYS	SER	GLY	TYR	PRO	ASN	MET	ASP	LEU	ASP
24	CYS	LEU	LEU	PRO	GLN	GLU	TRP	VAL	GLN	HIS
25	PHE	TYR	GLN	GLU	ALA	ALA	PRO	ALA	GLN	SER
26	ASP	VAL	ALA	LEU	LEU	ARG	PHE	VAL	ASN	PRO
27	ASP	THR	GLY	ARG	VAL	LEU	PHE	GLU	CYS	LYS
28	LEU	HIS	LYS	SER	GLY	TYR	VAL	THR	VAL	ALA
29	HIS	THR	GLY	GLN	HIS	ASP	LEU	VAL	ILE	PRO
30	PRO	ASN	GLY	TYR	PHE	ARG	PHE	ASP	SER	TRP
31	VAL	ASN	GLN	PHE	TYR	THR	LEU	ALA	PRO	MET

1	GLY	PRO	GLY	SER	LYS	PRO	PHE	THR	VAL	PRO
2	ILE	LEU	THR	VAL	GLU	GLU	MET	THR	ASN	SER
3	ARG	PHE	PRO	ILE	PRO	LEU	GLU	LYS	LEU	PHE
4	THR	GLY	PRO	SER	GLY	ALA	PHE	VAL	VAL	GLN
5	PRO	GLN	ASN	GLY	ARG	CYS	THR	THR	ASP	GLY
6	VAL	LEU	LEU	GLY	THR	THR	GLN	LEU	SER	PRO
7	VAL	ASN	ILE	CYS	THR	PHE	ARG	GLY	ASP	VAL
8	THR	HIS	ILE	ALA	GLY	SER	ARG	ASN	TYR	THR
9	MET	ASN	LEU	ALA	SER	LEU	ASN	TRP	ASN	ASN
10	TYR	ASP	PRO	THR	GLU	GLU	ILE	PRO	ALA	PRO
11	LEU	GLY	THR	PRO	ASP	PHE	VAL	GLY	LYS	ILE
12	GLN	GLY	LEU	LEU	THR	GLN	THR	THR	LYS	GLY
13	ASP	GLY	SER	THR	ARG	GLY	HIS	LYS	ALA	THR
14	VAL	TYR	THR	GLY	SER	ALA	PRO	PHE	THR	PRO
15	LYS	LEU	GLY	SER	VAL	GLN	PHE	SER	THR	ASP
16	THR	GLU	ASN	ASP	PHE	GLU	THR	HIS	GLN	ASN
17	THR	LYS	PHE	THR	PRO	VAL	GLY	VAL	ILE	GLN
18	ASP	GLY	SER	THR	THR	HIS	ARG	ASN	GLU	PRO
19	GLN	GLN	TRP	VAL	LEU	PRO	SER	TYR	SER	GLY
20	ARG	ASN	VAL	HIS	ASN	VAL	HIS	LEU	ALA	PRO
21	ALA	VAL	ALA	PRO	THR	PHE	PRO	GLY	GLU	GLN
22	LEU	LEU	PHE	PHE	ARG	SER	THR	MET	PRO	GLY
23	CYS	SER	GLY	TYR	PRO	ASN	MET	ASP	LEU	ASP
24	CYS	LEU	LEU	PRO	GLN	GLU	TRP	VAL	GLN	HIS
25	PHE	TYR	GLN	GLU	ALA	ALA	PRO	ALA	GLN	SER
26	ASP	VAL	ALA	LEU	LEU	ARG	PHE	VAL	ASN	PRO
27	ASP	THR	GLY	ARG	VAL	LEU	PHE	GLU	CYS	LYS
28	LEU	HIS	LYS	SER	GLY	TYR	VAL	THR	VAL	ALA
29	HIS	THR	GLY	GLN	HIS	ASP	LEU	VAL	ILE	PRO
30	PRO	ASN	GLY	TYR	PHE	ARG	PHE	ASP	SER	TRP
31	VAL	ASN	GLN	PHE	TYR	THR	LEU	ALA	PRO	MET