BMRB Entry 27436

Title:
Backbone and side-chain resonance assignments of the methyl-CpG-binding domain of MBD6 from Arabidopsis thaliana
Deposition date:
2018-03-28
Original release date:
2018-11-16
Authors:
Iwakawa, Naoto; Mahana, Yutaka; Ono, Arina; Ohki, Izuru; Walinda, Erik; Morimoto, Daichi; Sugase, Kenji; Shirakawa, Masahiro
Citation:

Citation: Iwakawa, Naoto; Mahana, Yutaka; Ono, Arina; Ohki, Izuru; Walinda, Erik; Morimoto, Daichi; Sugase, Kenji; Shirakawa, Masahiro. "Backbone and side-chain resonance assignments of the methyl-CpG-binding domain of MBD6 from Arabidopsis thaliana"  Biomol. NMR Assignments 13, 59-62 (2019).
PubMed: 30242623

Assembly members:

Assembly members:
AtMBD6, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6p-1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts64
1H chemical shifts454

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBD61

Entities:

Entity 1, MBD6 68 residues - Formula weight is not available

1   GLYPROLEUGLYSERASPASNTRPLEUPRO
2   PROGLYTRPARGVALGLUASPLYSILEARG
3   THRSERGLYALATHRALAGLYSERVALASP
4   LYSTYRTYRTYRGLUPROASNTHRGLYARG
5   LYSPHEARGSERARGTHRGLUVALLEUTYR
6   TYRLEUGLUHISGLYTHRSERLYSARGGLY
7   THRLYSLYSALAGLUASNTHRTYR

Samples:

sample_1: AtMBD6, [U-100% 13C; U-100% 15N], 5 mM; D2O, [U-2H], 5%; H2O 95%; sodium chloride 50 mM; sodium phosphate 20 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

MagRO, Kobayashi N, Iwahara J, Koshiba S, Tomizawa T, Tochio N, G ntert P, Kigawa T, Yokoyama S - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks