BMRB Entry 27406

Title:
Backbone and side chain NMR assignments for heterologously expressed Er-23
Deposition date:
2018-02-18
Original release date:
2018-06-20
Authors:
Bowles, David; Hansen, Alexandar; Rhoads, Calvin; Yuan, Chunhua; Magliery, Thomas
Citation:

Citation: Bowles, David; Hansen, Alexandar; Rhoads, Calvin; Yuan, Chunhua; Magliery, Thomas. "1H, 13C, 15N resonance assignment of recombinant Euplotes raikovi protein Er-23"  Biomol. NMR Assignments 12, 291-295 (2018).
PubMed: 29876749

Assembly members:

Assembly members:
Er-23, polymer, 54 residues, 5300 Da.

Natural source:

Natural source:   Common Name: Euplotes raikovi   Taxonomy ID: 5938   Superkingdom: Eukaryota   Kingdom: Ciliophora   Genus/species: Euplotes raikovi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHLIC

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts112
15N chemical shifts56
1H chemical shifts249

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Er-231

Entities:

Entity 1, Er-23 54 residues - 5300 Da.

1   GLYSERSERGLYGLUCYSGLUGLNCYSPHE
2   SERASPGLYGLYASPCYSTHRTHRCYSPHE
3   ASNASNGLYTHRGLYPROCYSALAASNCYS
4   LEUALAGLYTYRPROALAGLYCYSSERASN
5   SERASPCYSTHRALAPHELEUSERGLNCYS
6   TYRGLYGLYCYS

Samples:

sample_1: Er-23, [U-100% 13C; U-100% 15N], 1 – 1.2 mM; Tris-HCl1 – 50 mM; Tris-HCl1 – 300 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Ascend 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks