BMRB Entry 27347

Title:
Babckbone 1HN, 13C, and 15N Chemical Shift Assignments of the ATAD2B Bromodomain
Deposition date:
2017-12-21
Original release date:
2021-07-22
Authors:
Cornilescu, Gabriel
Citation:

Citation: Lloyd, Jonathan; McLaughlin, Kyle; Lubula, Mulu; Gay, Jamie; Dest, Andrea; Gao, Cong; Phillips, Margaret; Tonelli, Marco; Cornilescu, Gabriel; Marunde, Matthew; Evans, Chiara; Boyson, Samuel; Carlson, Samuel; Keogh, Michael-Christopher; Markley, John; Frietze, Seth; Glass, Karen. "Structural Insights into the Recognition of Mono- and Diacetylated Histones by the ATAD2B Bromodomain"  J. Med. Chem. 63, 12799-12813 (2020).
PubMed: 33084328

Assembly members:

Assembly members:
ATAD2B_bromodomain, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST15

Data sets:
Data typeCount
13C chemical shifts225
15N chemical shifts118
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ATAD2B bromodomain1

Entities:

Entity 1, ATAD2B bromodomain 136 residues - Formula weight is not available

1   GLYPROLEUGLUASPGLNGLUGLUASNTHR
2   LEUARGGLULEUARGLEUPHELEUARGASP
3   VALTHRLYSARGLEUALATHRASPLYSARG
4   PHEASNILEPHESERLYSPROVALASPILE
5   GLUGLUVALSERASPTYRLEUGLUVALILE
6   LYSGLUPROMETASPLEUSERTHRVALILE
7   THRLYSILEASPLYSHISASNTYRLEUTHR
8   ALALYSASPPHELEULYSASPILEASPLEU
9   ILECYSSERASNALALEUGLUTYRASNPRO
10   ASPLYSASPPROGLYASPLYSILEILEARG
11   HISARGALACYSTHRLEULYSASPTHRALA
12   HISALAILEILEALAALAGLULEUASPPRO
13   GLUPHEASNLYSLEUCYSGLUGLUILELYS
14   GLUALAARGILELYSARG

Samples:

sample_1: ATAD2B bromodomain, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Agilent INOVA 600 MHz

Related Database Links:

UNP Q9ULI0
AlphaFold Q8N9E7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks