BMRB Entry 27336

Title:
N-terminal domain of FOXO1
Deposition date:
2017-12-07
Original release date:
2019-07-15
Authors:
Saline, Maria; Badertscher, Lukas; Gunnarsson, Anders; Snow, Melanie; Jacso, Tomas; Norris, Tyrrell; Snijder, Arjan
Citation:

Citation: Saline, Maria; Badertscher, Lukas; Wolter, Madita; Lau, Roxanne; Gunnarsson, Anders; Jacso, Tomas; Norris, Tyrrell; Ottmann, Christian; Snijder, Arjan. "AMPK and AKT protein kinases hierarchically phosphorylate the N-terminus of the FOXO1 transcription factor, modulating interactions with 14-3-3 proteins"  J. Biol. Chem. 294, 13106-13116 (2019).
PubMed: 31308176

Assembly members:

Assembly members:
transcription_factor_FOXO1, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
transcription_factor_FOXO1: MAHNHNHNHNHNHNENLYFQ GMAEAPQVVEIDPDFEPLPR PRSCTWPLPRPEFSQSNSAT SSPAPSG

Data sets:
Data typeCount
13C chemical shifts86
15N chemical shifts44
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1transcription factor FOXO11

Entities:

Entity 1, transcription factor FOXO1 67 residues - Formula weight is not available

Residues 1-21 represent a non-native affinity tag, followed by the initial 45 residues in FOXO1.

1   METALAHISASNHISASNHISASNHISASN
2   HISASNHISASNGLUASNLEUTYRPHEGLN
3   GLYMETALAGLUALAPROGLNVALVALGLU
4   ILEASPPROASPPHEGLUPROLEUPROARG
5   PROARGSERCYSTHRTRPPROLEUPROARG
6   PROGLUPHESERGLNSERASNSERALATHR
7   SERSERPROALAPROSERGLY

Samples:

FOXO1: transcription factor FOXO1, [U-98% 13C; U-98% 15N], 200 uM; potassium phosphate 20 mM; sodium chloride 100 mM; TCEP 1 mM; MgCl 10 mM; ATP 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCFOXO1isotropicsample_conditions_1
3D HNCACBFOXO1isotropicsample_conditions_1
3D HNCAFOXO1isotropicsample_conditions_1
3D HN(COCA)CBFOXO1isotropicsample_conditions_1

Software:

TOPSPIN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks