BMRB Entry 27315

Title:
Outer Membrane Protein X in PC14:1 MSPdH5 Nanodisc
Deposition date:
2017-11-22
Original release date:
2018-12-10
Authors:
Frey, Lukas; Riek, Roland; Bibow, Stefan
Citation:

Citation: Frey, Lukas; Hiller, Sebastian; Riek, Roland; Bibow, Stefan. "Lipid- and Cholesterol-Mediated Time-Scale-Specific Modulation of the Outer Membrane Protein X Dynamics in Lipid Bilayers"  J. Am. Chem. Soc. 140, 15402-15411 (2018).
PubMed: 30289706

Assembly members:

Assembly members:
MSPdH5, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_28b

Data sets:
Data typeCount
13C chemical shifts141
15N chemical shifts144
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MSPdH5 Nanodisc1

Entities:

Entity 1, MSPdH5 Nanodisc 148 residues - Formula weight is not available

1   ALATHRSERTHRVALTHRGLYGLYTYRALA
2   GLNSERASPALAGLNGLYGLNMETASNLYS
3   METGLYGLYPHEASNLEULYSTYRARGTYR
4   GLUGLUASPASNSERPROLEUGLYVALILE
5   GLYSERPHETHRTYRTHRGLULYSSERARG
6   THRALASERSERGLYASPTYRASNLYSASN
7   GLNTYRTYRGLYILETHRALAGLYPROALA
8   TYRARGILEASNASPTRPALASERILETYR
9   GLYVALVALGLYVALGLYTYRGLYLYSPHE
10   GLNTHRTHRGLUTYRPROTHRTYRLYSASN
11   ASPTHRSERASPTYRGLYPHESERTYRGLY
12   ALAGLYLEUGLNPHEASNPROMETGLUASN
13   VALALALEUASPPHESERTYRGLUGLNSER
14   ARGILEARGSERVALASPVALGLYTHRTRP
15   ILEALAGLYVALGLYTYRARGPHE

Samples:

sample_1: OmpX in MSPdH5 Nanodisc, [U-100% 13C; U-100% 15N], 300 uM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 299.5 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Cambridge Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks