BMRB Entry 27198

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Dm. Par3 PDZ3 domain
Deposition date:
2017-08-02
Original release date:
2018-02-15
Authors:
Susanne, Bruekner; Fabian, Renschler; Silke, Wiesner
Citation:

Citation: Renschler, Fabian; Bruekner, Susanne; Salomon, Paulin; Mukherjee, Amrita; Kullmann, Lars; Schutz-Stoffregen, Mira; Henzler, Christine; Pawson, Tony; Krahn, Michael; Wiesner, Silke. "Structural basis for the interaction between the cell polarity proteins Par3 and Par6"  Sci. Signal. 11, 9899-9899 (2018).
PubMed: 29440511

Assembly members:

Assembly members:
Par3_PDZ3_domain, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-30

Data sets:
Data typeCount
13C chemical shifts268
15N chemical shifts89
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Par3 PDZ3 domain1

Entities:

Entity 1, Par3 PDZ3 domain 108 residues - Formula weight is not available

Residues 667-685 of the natural sequence were deleted from the domain

1   ASNSERTRPHISSERARGGLUGLULEUTHR
2   LEUHISILEPROVALHISASPTHRGLULYS
3   ALAGLYLEUGLYVALSERVALLYSGLYLYS
4   THRCYSSERASPGLYASPLEUGLYILEPHE
5   VALLYSASNVALILEHISGLYGLYALAALA
6   SERARGASPGLYARGLEUARGMETASNASP
7   GLNLEULEUSERVALASNGLYVALSERLEU
8   ARGGLYGLNASNASNALAGLUALAMETGLU
9   THRLEUARGARGALAMETVALASNTHRPRO
10   GLYLYSHISPROGLYTHRILETHRLEULEU
11   VALGLYARGLYSILELEUARGSER

Samples:

sample_1: Par3 PDZ3 domain, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks