BMRB Entry 27160

Title:
1H, 15N, 13C backbone resonance assignments of the nuclease core residues 2-336 of wild type human flap endonuclease-1
Deposition date:
2017-06-23
Original release date:
2018-05-22
Authors:
Bennet, Ian; Baxter, Nicola
Citation:

Citation: Bennet, Ian; Finger, L David; Baxter, Nicola; Ambrose, Benjamin; Hounslow, Andrea; Thompson, Mark; Exell, Jack; Shahari, Nur Nazihah; Craggs, Timothy; Waltho, Jonathan; Grasby, Jane. "Regional conformational flexibility couples substrate specificity and scissile phosphate diester selectivity in human flap endonuclease 1"  Nucleic Acids Res. 46, 5618-5633 (2018).
PubMed: 29718417

Assembly members:

Assembly members:
hFEN1, polymer, 341 residues, 38457.18 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-29b

Data sets:
Data typeCount
13C chemical shifts797
15N chemical shifts247
1H chemical shifts247

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hFEN1-3361

Entities:

Entity 1, hFEN1-336 341 residues - 38457.18 Da.

1   GLYILEGLNGLYLEUALALYSLEUILEALA
2   ASPVALALAPROSERALAILEARGGLUASN
3   ASPILELYSSERTYRPHEGLYARGLYSVAL
4   ALAILEASPALASERMETSERILETYRGLN
5   PHELEUILEALAVALARGGLNGLYGLYASP
6   VALLEUGLNASNGLUGLUGLYGLUTHRTHR
7   SERHISLEUMETGLYMETPHETYRARGTHR
8   ILEARGMETMETGLUASNGLYILELYSPRO
9   VALTYRVALPHEASPGLYLYSPROPROGLN
10   LEULYSSERGLYGLULEUALALYSARGSER
11   GLUARGARGALAGLUALAGLULYSGLNLEU
12   GLNGLNALAGLNALAALAGLYALAGLUGLN
13   GLUVALGLULYSPHETHRLYSARGLEUVAL
14   LYSVALTHRLYSGLNHISASNASPGLUCYS
15   LYSHISLEULEUSERLEUMETGLYILEPRO
16   TYRLEUASPALAPROSERGLUALAGLUALA
17   SERCYSALAALALEUVALLYSALAGLYLYS
18   VALTYRALAALAALATHRGLUASPMETASP
19   CYSLEUTHRPHEGLYSERPROVALLEUMET
20   ARGHISLEUTHRALASERGLUALALYSLYS
21   LEUPROILEGLNGLUPHEHISLEUSERARG
22   ILELEUGLNGLULEUGLYLEUASNGLNGLU
23   GLNPHEVALASPLEUCYSILELEULEUGLY
24   SERASPTYRCYSGLUSERILEARGGLYILE
25   GLYPROLYSARGALAVALASPLEUILEGLN
26   LYSHISLYSSERILEGLUGLUILEVALARG
27   ARGLEUASPPROASNLYSTYRPROVALPRO
28   GLUASNTRPLEUHISLYSGLUALAHISGLN
29   LEUPHELEUGLUPROGLUVALLEUASPPRO
30   GLUSERVALGLULEULYSTRPSERGLUPRO
31   ASNGLUGLUGLULEUILELYSPHEMETCYS
32   GLYGLULYSGLNPHESERGLUGLUARGILE
33   ARGSERGLYVALLYSARGLEUSERLYSSER
34   ARGGLNGLYSERTHRLEUGLUVALLEUPHE
35   GLN

Samples:

sample_1: hFEN1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; HEPES 10 mM; potassium chloride 76 mM; EDTA 0.1 mM; sodium azide 4 mM; D2O, [U-99% 2H], 10%; H2O 90%; TSP, [U-99% 2H], 0.05 mM; beta-mercaptoethanol 100 mM

sample_conditions_1: ionic strength: 90 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D TROSY-HNCAsample_1isotropicsample_conditions_1
3D TROSY-HN(CO)CAsample_1isotropicsample_conditions_1
3D TROSY-HNCACBsample_1isotropicsample_conditions_1
3D TROSY-HN(CO)CACBsample_1isotropicsample_conditions_1
3D TROSY-HN(CA)COsample_1isotropicsample_conditions_1
3D TROSY-HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

Felix, Accelrys Software Inc. - chemical shift assignment, data analysis, peak picking, processing

CCPN_Analysis, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks