BMRB Entry 27044

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sgt2_TPR
Deposition date:
2017-03-06
Original release date:
2017-11-10
Authors:
Krysztofinska, Ewelina; Evans, Nicola; Thapaliya, Arjun; Murray, James; Morgan, Rhodri; Martinez-Lumbreras, Santiago; Isaacson, Rivka
Citation:

Citation: Krysztofinska, Ewelina; Evans, Nicola; Thapaliya, Arjun; Murray, James; Morgan, Rhodri; Martinez-Lumbreras, Santiago; Isaacson, Rivka. "Structure and Interactions of the TPR Domain of Sgt2 with Yeast Chaperones and Ybr137wp."  Front Mol Biosci 4, .-. (2017).
PubMed: 29075633

Assembly members:

Assembly members:
Sgt2, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet28

Data sets:
Data typeCount
13C chemical shifts407
15N chemical shifts135
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sgt2_TPR monomer1

Entities:

Entity 1, Sgt2_TPR monomer 140 residues - Formula weight is not available

1   GLYSERMETGLUASPASPALAGLUTHRLYS
2   ALALYSALAGLUASPLEULYSMETGLNGLY
3   ASNLYSALAMETALAASNLYSASPTYRGLU
4   LEUALAILEASNLYSTYRTHRGLUALAILE
5   LYSVALLEUPROTHRASNALAILETYRTYR
6   ALAASNARGALAALAALAHISSERSERLEU
7   LYSGLUTYRASPGLNALAVALLYSASPALA
8   GLUSERALAILESERILEASPPROSERTYR
9   PHEARGGLYTYRSERARGLEUGLYPHEALA
10   LYSTYRALAGLNGLYLYSPROGLUGLUALA
11   LEUGLUALATYRLYSLYSVALLEUASPILE
12   GLUGLYASPASNALATHRGLUALAMETLYS
13   ARGASPTYRGLUSERALALYSLYSLYSVAL
14   GLUGLNSERLEUASNLEUGLULYSTHRVAL

Samples:

sample_1: Sgt2, [U-13C; U-15N], 0.5 mM; potassium phosphate 10 mM; sodium chloride 100 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 0.010 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks