BMRB Entry 27034

Title:
1H, 13C and 15N chemical shift assignments of A. thaliana RCD1(468-569)
Deposition date:
2017-02-13
Original release date:
2017-06-13
Authors:
Tossavainen, Helena; Hellman, Maarit; Vainonen, Julia; Kangasjarvi, Jaakko; Permi, Perttu
Citation:

Citation: Tossavainen, Helena; Hellman, Maarit; Vainonen, Julia; Kangasjarvi, Jaakko; Permi, Perttu. "1H, 13C and 15N NMR chemical shift assignments of A. thaliana RCD1 RST"  Biomol. NMR Assignments 11, 207-210 (2017).
PubMed: 28593560

Assembly members:

Assembly members:
RCD1, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T-1

Data sets:
Data typeCount
13C chemical shifts500
15N chemical shifts123
1H chemical shifts811

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RCD11

Entities:

Entity 1, RCD1 128 residues - Formula weight is not available

1   GLYSERPROGLUPHEMETGLYVALTHRLEU
2   GLUGLYPROLYSASPLEUPROPROGLNLEU
3   GLUSERASNGLNGLYALAARGGLYSERGLY
4   SERALAASNSERVALGLYSERSERTHRTHR
5   ARGPROLYSSERPROTRPMETPROPHEPRO
6   THRLEUPHEALAALAILESERHISLYSVAL
7   ALAGLUASNASPMETLEULEUILEASNALA
8   ASPTYRGLNGLNLEUARGASPLYSLYSMET
9   THRARGALAGLUPHEVALARGLYSLEUARG
10   VALILEVALGLYASPASPLEULEUARGSER
11   THRILETHRTHRLEUGLNASNGLNPROLYS
12   SERLYSGLUILEPROGLYSERILEARGASP
13   HISGLUGLUGLYALAGLYGLYLEU

Samples:

sample_1: RCD1, [U-13C; U-15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D iHNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D hCCmHmsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks