BMRB Entry 27029

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignment for Human TRPV1 Sensing Domain
Deposition date:
2017-02-09
Original release date:
2021-11-17
Authors:
Kim, Minjoo; Sisco, Nicholas; Van Horn, Wade
Citation:

Citation: Kim, Minjoo; Sisco, Nicholas; Hilton, Jacob; Montano, Camila; Castro, Manuel; Cherry, Brian; Levitus, Marcia; Van Horn, Wade. "Evidence that the TRPV1 S1-S4 membrane domain contributes to thermosensing"  Nat. Commun. 11, 4169-4169 (2020).
PubMed: 32820172

Assembly members:

Assembly members:
hV1-SD, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Data sets:
Data typeCount
13C chemical shifts328
15N chemical shifts118
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hV1-SD monomer1

Entities:

Entity 1, hV1-SD monomer 143 residues - Formula weight is not available

1   GLYSERPROLEUASNARGLEULEUGLNASP
2   LYSTRPASPARGPHEVALLYSARGILEPHE
3   TYRPHEASNPHELEUVALTYRCYSLEUTYR
4   METILEILEPHETHRMETALAALATYRTYR
5   ARGPROVALASPGLYLEUPROPROPHELYS
6   METGLULYSTHRGLYASPTYRPHEARGVAL
7   THRGLYGLUILELEUSERVALLEUGLYGLY
8   VALTYRPHEPHEPHEARGGLYILEGLNTYR
9   PHELEUGLNARGARGPROSERMETLYSTHR
10   LEUPHEVALASPSERTYRSERGLUMETLEU
11   PHEPHELEUGLNSERLEUPHEMETLEUALA
12   THRVALVALLEUTYRPHESERHISLEULYS
13   GLUTYRVALALASERMETVALPHESERLEU
14   ALALEUGLYTRPTHRASNMETLEUTYRTYR
15   THRARGGLY

Samples:

sample_1: hV1-SD, [U-100% 13C; U-100% 15N], 0.9 mM; LPPG 2.5 % w/v; sodium phosphate 2.5 mM; EDTA 0.5 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

UNP Q8NER1
AlphaFold Q9NY22

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks