BMRB Entry 27019

Title:
RXFP2-LDLa-linker-GB1
Deposition date:
2017-01-31
Original release date:
2018-09-04
Authors:
Sethi, Ashish; Gooley, Paul
Citation:

Citation: Bruell, Shoni; Sethi, Ashish; Smith, Nicholas; Scott, Daniel; Hossain, Mohammed Akhter; Wu, Qing-Ping; Guo, Zhan-Yun; Petrie, Emma; Gooley, Paul; Bathgate, Ross. "The involvement of the LDLa linker in the mode of activation of the GPCR RXFP2"  .

Assembly members:

Assembly members:
RXFP2-LDLa-linker-GB1, polymer, 121 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts119
1H chemical shifts566

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RXFP2-LDLa-linker-GB11
2Calcium2

Entities:

Entity 1, RXFP2-LDLa-linker-GB1 121 residues - Formula weight is not available

1   METILETHRPHESERCYSGLNLYSGLYTYR
2   PHEPROCYSGLYASNLEUTHRLYSCYSLEU
3   PROARGALAPHEHISCYSASPGLYLYSASP
4   ASPCYSGLYASNGLYALAASPGLUGLUASN
5   CYSGLYASPTHRSERGLYTRPALATHRILE
6   PHEGLYTHRVALHISGLYASNALAASNSER
7   VALALALEUTHRGLNGLUGLNTYRLYSLEU
8   ALALEUASNGLYLYSTHRLEULYSGLYGLU
9   THRTHRTHRGLUALAVALASPALAALATHR
10   ALAGLULYSVALPHELYSGLNTYRALAASN
11   ASPASNGLYVALASPGLYGLUTRPTHRTYR
12   ASPASPALATHRLYSTHRPHETHRVALTHR
13   GLU

Entity 2, Calcium - Ca - 40.078 Da.

1   CA

Samples:

sample_1: RXFP2-LDLa-linker-GB1, [U-100% 15N], 300 uM; RXFP2-LDLa-linker-GB1, [U-100% 13C; U-100% 15N], 350 uM

sample_conditions_1: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks