BMRB Entry 26995

Title:
A consensus homeodomain
Deposition date:
2017-01-15
Original release date:
2017-03-31
Authors:
Sternke, Matt; Barrick, Doug; Tripp, Katherine; Majumdar, Ananya
Citation:

Citation: Tripp, Katherine; Sternke, Matt; Majumdar, Ananya; Barrick, Doug; Tripp, Katherine; Sternke, Matt; Majumdar, Ananya; Barrick, Doug. "Assigned Chemical Shifts for a consensus homeodomain"  .

Assembly members:

Assembly members:
Consensus_homeomdomain, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Consensus_homeomdomain: MGSRRKRTTFTKEQLEELEE LFEKNRYPSAEEREELAKKL GLTERQVKVWFQNRRAKEKK HHHHHH

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts55
1H chemical shifts375

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Consensus homeomdomain, subunit 11
2Consensus homeomdomain, subunit 21

Entities:

Entity 1, Consensus homeomdomain, subunit 1 66 residues - Formula weight is not available

1   METGLYSERARGARGLYSARGTHRTHRPHE
2   THRLYSGLUGLNLEUGLUGLULEUGLUGLU
3   LEUPHEGLULYSASNARGTYRPROSERALA
4   GLUGLUARGGLUGLULEUALALYSLYSLEU
5   GLYLEUTHRGLUARGGLNVALLYSVALTRP
6   PHEGLNASNARGARGALALYSGLULYSLYS
7   HISHISHISHISHISHIS

Samples:

sample_1: Consensus homeomdomain, [U-100% 13C; U-100% 15N], 400 uM; potassium phosphate 25 mM; potassium chloride 150 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCCO)NH-TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)Nsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks