BMRB Entry 26929

Title:
1H and 15N resonance assignment of the first fibronectin type III module of the neural cell adhesion molecule (NCAM FN1)
Deposition date:
2016-10-28
Original release date:
2017-04-26
Authors:
Bhide, Gaurang; Prehna, Gerd; Ramirez, Benjamin; Colley, Karen
Citation:

Citation: Bhide, Gaurang; Prehna, Gerd; Ramirez, Benjamin; Colley, Karen. "The Polybasic Region of the Polysialyltransferase ST8Sia-IV Binds Directly to the Neural Cell Adhesion Molecule, NCAM"  Biochemistry 56, 1504-1517 (2017).
PubMed: 28233978

Assembly members:

Assembly members:
NCAM_FN1, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14(b)

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts98
1H chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NCAM FN11

Entities:

Entity 1, NCAM FN1 127 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLNALAASPTHRPROSERSERPROSER
4   ILEASPGLNVALGLUPROTYRSERSERTHR
5   ALAGLNVALGLNPHEASPGLUPROGLUALA
6   THRGLYGLYVALPROILELEULYSTYRLYS
7   ALAGLUTRPARGALAVALGLYGLUGLUVAL
8   TRPHISSERLYSTRPTYRASPALALYSGLU
9   ALASERMETGLUGLYILEVALTHRILEVAL
10   GLYLEULYSPROGLUTHRTHRTYRALAVAL
11   ARGLEUALAALALEUASNGLYLYSGLYLEU
12   GLYGLUILESERALAALASERGLUPHELYS
13   THRGLNPROVALGLNGLYGLU

Samples:

sample_1: NCAM FN1, [U-99% 13C; U-99% 15N], 500 mM; potassium phosphate 20 mM; sodium chloride 300 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.3 M; pH: 6.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks