BMRB Entry 26921

Title:
Chemical Shift Assignments of the Connexin37 Carboxyl Terminal Domain
Deposition date:
2016-10-21
Original release date:
2017-05-23
Authors:
Li, Hanjun; Spagnol, Gaelle; Nelson, Tasha; Burt, Janis; Sorgen, Paul
Citation:

Citation: Li, Hanjun; Spagnol, Gaelle; Pontifex, Tasha; Burt, Janis; Spagnol, Paul. "Chemical shift assignments of the connexin37 carboxyl terminal domain"  Biomol. NMR Assignments 11, 137-141 (2017).
PubMed: 28251507

Assembly members:

Assembly members:
Cx37CT_Domain, polymer, 103 residues, 11257.41 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-KT

Data sets:
Data typeCount
13C chemical shifts397
15N chemical shifts88
1H chemical shifts632

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cx37CT Domain1

Entities:

Entity 1, Cx37CT Domain 103 residues - 11257.41 Da.

Residues 1 and 2 represent part of the remaining non-native affinity tag. This is the C-terminal cytoplasmic domain of a membrane protein.

1   GLYSERCYSARGCYSVALSERARGGLUILE
2   LYSALAARGARGASPHISASPALAARGPRO
3   ALAGLNGLYSERALASERASPPROTYRPRO
4   GLUGLNVALPHEPHETYRLEUPROMETGLY
5   GLUGLYPROSERSERPROPROCYSPROTHR
6   TYRASNGLYLEUSERSERTHRGLUGLNASN
7   TRPALAASNLEUTHRTHRGLUGLUARGLEU
8   THRSERSERARGPROPROPROPHEVALASN
9   THRALAPROGLNGLYGLYARGLYSSERPRO
10   SERARGPROASNSERSERALASERLYSLYS
11   GLNTYRVAL

Samples:

sample_1: Cx37CT Domain, [U-99% 13C; U-99% 15N], 650 uM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; potassium chloride 2.7 mM; sodium chloride 137 mM; DTT 2 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 0.154 M; pH: 5.8; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView v5.2.2 01, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks