BMRB Entry 26900

Title:
Beta-endorphin salt-free amyloid fibrils
Deposition date:
2016-09-16
Original release date:
2021-07-22
Authors:
Seuring, Carolin; Verasdonck, Joeri; Ringler, Philippe; Cadalbert, Riccardo; Stahlberg, Henning; Boeckmann, Anja; Meier, Beat; Riek, Roland
Citation:

Citation: Seuring, Carolin; Verasdonck, Joeri; Ringler, Philippe; Cadalbert, Riccardo; Stahlberg, Henning; Boeckmann, Anja; Meier, Beat; Riek, Roland. "Amyloid Fibril Polymorphism: Almost Identical on the Atomic Level, Mesoscopically Very Different"  J Phys Chem B 121, 1783-1792 (2017).
PubMed: 28075583

Assembly members:

Assembly members:
beta-endorphin, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-endorphin: YGGFMTSEKSQTPLVTLFKN AIIKNAYKKE

Data sets:
Data typeCount
13C chemical shifts122
15N chemical shifts28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta-endorphin amyloid fibril1

Entities:

Entity 1, beta-endorphin amyloid fibril 30 residues - Formula weight is not available

1   TYRGLYGLYPHEMETTHRSERGLULYSSER
2   GLNTHRPROLEUVALTHRLEUPHELYSASN
3   ALAILEILELYSASNALATYRLYSLYSGLU

Samples:

sample_1: beta-endorphin, [U-100% 13C; U-100% 15N], 25 mg

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 20 ms DARsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
3D NCOCAsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1

Software:

TOPSPIN, CCPN - collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 600 MHz