BMRB Entry 26854

Name: AsLOV2 (Avena sativa phototropin 1, LOV2 domain)
Published: 2016-08-11

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26854

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

AsLOV2 (Avena sativa phototropin 1, LOV2 domain)

Deposition date:

2016-07-14

Original release date:

2016-08-11

Authors:

Gardner, Kevin

Citation:

Citation: Harper, Shannon; Neil, Lori; Gardner, Kevin. "Structural basis of a phototropin light switch" Science 301, 1541-1544 (2003).
PubMed: 12970567

Assembly members:

Assembly members:
AsLOV2, polymer, 161 residues, Formula weight is not available
FLAVIN MONONUCLEOTIDE, non-polymer, 456.344 Da.

Natural source:

Natural source: Common Name: oat Taxonomy ID: 4498 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Avena sativa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis-GB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AsLOV2: GEFLATTLERIEKNFVITDP RLPDNPIIFASDSFLQLTEY SREEILGRNCRFLQGPETDR ATVRKIRDAIDNQTEVTVQL INYTKSGKKFWNLFHLQPMR DQKGDVQYFIGVQLDGTEHV RDAAEREGVMLIKKTAENID EAAKELPDANLRPEDLWANH G

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	644
15N chemical shifts	166
1H chemical shifts	987

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AsLOV2 protein	1
2	AsLOV2 FMN	2

Entities:

Entity 1, AsLOV2 protein 161 residues - Formula weight is not available

residues 1-3 (GEF) and residue 161 (G) are vector-derived residues 4-160 correspond to residues 404-560 of A. sativa phototropin 1

1

GLY

GLU

PHE

LEU

ALA

THR

LEU

GLU

ARG

2

ILE

GLU

LYS

ASN

PHE

VAL

ILE

THR

ASP

PRO

3

ARG

LEU

PRO

ASP

ASN

PRO

ILE

PHE

ALA

4

SER

ASP

SER

PHE

LEU

GLN

LEU

THR

GLU

TYR

5

SER

ARG

GLU

ILE

LEU

GLY

ARG

ASN

CYS

6

ARG

PHE

LEU

GLN

GLY

PRO

GLU

THR

ASP

ARG

7

ALA

THR

VAL

ARG

LYS

ILE

ARG

ASP

ALA

ILE

8

ASP

ASN

GLN

THR

GLU

VAL

THR

VAL

GLN

LEU

9

ILE

ASN

TYR

THR

LYS

SER

GLY

LYS

PHE

10

TRP

ASN

LEU

PHE

HIS

LEU

GLN

PRO

MET

ARG

11

ASP

GLN

LYS

GLY

ASP

VAL

GLN

TYR

PHE

ILE

12

GLY

VAL

GLN

LEU

ASP

GLY

THR

GLU

HIS

VAL

13

ARG

ASP

ALA

GLU

ARG

GLU

GLY

VAL

MET

14

LEU

ILE

LYS

THR

ALA

GLU

ASN

ILE

ASP

15

GLU

ALA

LYS

GLU

LEU

PRO

ASP

ALA

ASN

16

LEU

ARG

PRO

GLU

ASP

LEU

TRP

ALA

ASN

HIS

17

GLY

Entity 2, AsLOV2 FMN - C17 H21 N4 O9 P - 456.344 Da.

1

FMN

Samples:

sample_1: AsLOV2, [U-100% 13C; U-100% 15N], 0.1 – 0.5 mM; sodium phosphate 50 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 500 MHz