BMRB Entry 26789

Name: 1H, 13C and 15N resonance assignments for the response regulator CheY3 from Rhodobacter sphaeroides at pH 7.3 in the absence of Mg2+
Published: 2016-09-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26789

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments for the response regulator CheY3 from Rhodobacter sphaeroides at pH 7.3 in the absence of Mg2+

Deposition date:

2016-04-26

Original release date:

2016-09-02

Authors:

Varela-Alvarez, Lorena; Bell, Christian; Armitage, Judith; Redfield, Christina

Citation:

Citation: Varela-Alvarez, Lorena; Bell, Christian; Armitage, Judith; Redfield, Christina. "1H, 13C and 15N resonance assignments for the response regulator CheY3 from Rhodobacter sphaeroides" Biomol. NMR Assign. 10, 373-378 (2016).
PubMed: 27468962

Assembly members:

Assembly members:
CheY3_ph7.3_nomg, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: a-proteobacteria Taxonomy ID: 1063 Superkingdom: Bacteria Kingdom: not available Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE-80

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CheY3_ph7.3_nomg: GSRTVLAVDDSPSVRSMVAM TLREAGYRVIEATDGREGLE KALSEPVDAIITDQNMPNLD GLGFIRAFREHPESKGKPII FLSTDSADTLKQQAREAGAM GWMVKPFTQPQLLAVIKKVL G

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	421
15N chemical shifts	106
1H chemical shifts	672

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CheY3_ph7.3_nomg	1

Entities:

Entity 1, CheY3_ph7.3_nomg 121 residues - Formula weight is not available

1

GLY

SER

ARG

THR

VAL

LEU

ALA

VAL

ASP

2

SER

PRO

SER

VAL

ARG

SER

MET

VAL

ALA

MET

3

THR

LEU

ARG

GLU

ALA

GLY

TYR

ARG

VAL

ILE

4

GLU

ALA

THR

ASP

GLY

ARG

GLU

GLY

LEU

GLU

5

LYS

ALA

LEU

SER

GLU

PRO

VAL

ASP

ALA

ILE

6

ILE

THR

ASP

GLN

ASN

MET

PRO

ASN

LEU

ASP

7

GLY

LEU

GLY

PHE

ILE

ARG

ALA

PHE

ARG

GLU

8

HIS

PRO

GLU

SER

LYS

GLY

LYS

PRO

ILE

9

PHE

LEU

SER

THR

ASP

SER

ALA

ASP

THR

LEU

10

LYS

GLN

ALA

ARG

GLU

ALA

GLY

ALA

MET

11

GLY

TRP

MET

VAL

LYS

PRO

PHE

THR

GLN

PRO

12

GLN

LEU

ALA

VAL

ILE

LYS

VAL

LEU

13

GLY

Samples:

sample_1: CheY3_ph7.3_nomg, [U-99% 13C; U-99% 15N], 0.7 mM; TRIS 50 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 7.3; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCA(CO)N	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpN_Analysis, CCPN - data analysis

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks