BMRB Entry 26706

Name: Backbone 13C, and 15 N Chemical Shift Assignments for fibrillar Islet Amyloid Polypeptide (IAPP)
Published: 2016-08-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26706

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 13C, and 15 N Chemical Shift Assignments for fibrillar Islet Amyloid Polypeptide (IAPP)

Deposition date:

2015-11-12

Original release date:

2016-08-08

Authors:

Weirich, Franziska; Heise, Henrike; Mirecka, Ewa; Gremer, Lothar; Hoyer, Wolfgang; Schiefer, Stephanie

Citation:

Citation: Weirich, Franziska; Mirecka, Ewa; Schiefer, Stephanie; Gremer, Lothar; Hoyer, Wolfgang; Heise, Henrike. "Structural Characterization of Fibrils from Recombinant Human Islet Amyloid Polypeptide by Solid-State NMR: The Central FGAILS Segment Is Part of the beta-Sheet Core" PLOS One 11, e0161243-e0161243 (2016).
PubMed: 27607147

Assembly members:

Assembly members:
IAPP, polymer, 37 residues, 3906.3 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET302/NT-His

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IAPP: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	109
15N chemical shifts	37

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	recombinant fibrillar hIAPP	1

Entities:

Entity 1, recombinant fibrillar hIAPP 37 residues - 3906.3 Da.

Full length human IAPP.

1

LYS

CYS

ASN

THR

ALA

THR

CYS

ALA

THR

GLN

2

ARG

LEU

ALA

ASN

PHE

LEU

VAL

HIS

SER

3

ASN

PHE

GLY

ALA

ILE

LEU

SER

THR

4

ASN

VAL

GLY

SER

ASN

THR

TYR

Samples:

sample_1: IAPP, [U-100% 13C; U-100% 15N], 110 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C PDSD	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
3D NCaCB	sample_1	isotropic	sample_conditions_1
3D NCaCX	sample_1	isotropic	sample_conditions_1
1D INEPT	sample_1	isotropic	sample_conditions_1
2D DQSQ SPC5_3	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2, CCPN - chemical shift assignment

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Oxford AS600 600 MHz
Varian 600/54 Premium Shielded 600 MHz
Oxford 2.2K 800/63 800 MHz