BMRB Entry 26617

Title:
Backbone Assignments for non-selective ion channel NaK tetramer
Deposition date:
2015-07-24
Original release date:
2015-12-18
Authors:
Brettmann, Joshua; Henzler-Wildman, Katherine
Citation:

Citation: Brettmann, Joshua; Urusova, Darya; Tonelli, Marco; Silva, Jonathan; Henzler-Wildman, Katherine. "Role of protein dynamics in ion selectivity and allosteric coupling in the NaK channel"  Proc. Natl. Acad. Sci. U.S.A. 112, 15366-15371 (2015).
PubMed: 26621745

Assembly members:

Assembly members:
NaK, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1396   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus cereus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet15B

Data sets:
Data typeCount
13C chemical shifts199
15N chemical shifts77
1H chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NaK, chain 11
2NaK, chain 21
3NaK, chain 31
4NaK, chain 41

Entities:

Entity 1, NaK, chain 1 96 residues - Formula weight is not available

1   GLYSERHISMETALALYSASPLYSGLUPHE
2   GLNVALLEUPHEVALLEUTHRILELEUTHR
3   LEUILESERGLYTHRILEPHETYRSERTHR
4   VALGLUGLYLEUARGPROILEASPALALEU
5   TYRPHESERVALVALTHRLEUTHRTHRVAL
6   GLYASPGLYASNPHESERPROGLNTHRASP
7   PHEGLYLYSILEPHETHRILELEUTYRILE
8   PHEILEGLYILEGLYLEUVALPHEGLYPHE
9   ILEHISLYSLEUALAVALASNVALGLNLEU
10   PROSERILELEUSERASN

Samples:

NaK_19: NaK, [U-100% 13C; U-100% 15N; U-70% 2H], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 40 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNaK_19isotropicsample_conditions_1
3D HNCONaK_19isotropicsample_conditions_1
3D HNCANaK_19isotropicsample_conditions_1
3D HNCACBNaK_19isotropicsample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DMX 750 MHz
  • Varian INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks