BMRB Entry 26377
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26377
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Sakurabayashi, Shuhei; Shinno, Yohei; Nakamachi, Ayano; Yoshiizumi, Yuki; Miyagawa, Koichi; Furuita, Kyoko; Shibata, Tomonori; Dohno, Chikara; Miyanoiri, Yohei; Nakatani, Kazuhiko; Kojima, Chojiro. "Structural basis for an asymmetric naphthyridine dimer that enhances binding to d(CGG/CGG)
" Nucleic Acids Res. ., .-..
Assembly members:
double-stranded DNA containing d(CGG/CGG) motif, polymer, 11 residues, Formula weight is not available
double-stranded DNA containing d(CGG/CGG) motif, polymer, 11 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: not available Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: unidentified
Entity Sequences (FASTA):
double-stranded DNA containing d(CGG/CGG) motif: CTAACGGAATG
double-stranded DNA containing d(CGG/CGG) motif: CATTCGGTTAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 83 |
| 15N chemical shifts | 10 |
| 1H chemical shifts | 147 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 1 | 1 |
| 2 | 2 | 2 |
Entities:
Entity 1, 1 11 residues - Formula weight is not available
| 1 | DC | DT | DA | DA | DC | DG | DG | DA | DA | DT | ||||
| 2 | DG |
Entity 2, 2 11 residues - Formula weight is not available
| 1 | DC | DA | DT | DT | DC | DG | DG | DT | DT | DA | ||||
| 2 | DG |
Samples:
sample_1: double-stranded DNA containing d(CGG/CGG) motif 2 mM; double-stranded DNA containing d(CGG/CGG) motif 2 mM; sodium chloride 100 mM; sodium phosphate 20 mM; H2O 95%; D2O, [U-2H], 5%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.7 - processing
NMRFAM-SPARKY v1.470 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
- Bruker AVANCE III 800 MHz