BMRB Entry 26374

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26374

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Title:
Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein, schizorhodopsin 1
Deposition date:
2025-09-19
Original release date:
2026-06-04
Authors:
Kitaguchi, Akito; Nagashima, Toshio; Kanazawa, Takumi; Yamazaki, Toshio; Inoue, Keiichi; Kawamura, Izuru
Citation:

Citation: Kitaguchi, Akito; Kanazawa, Takumi; Nagashima, Toshio; Yamazaki, Toshio; Okitsu, Takashi; Inoue, Keiichi; Kawamura, Izuru. "Site-resolved (13)C/(15)N solid-state nuclear magnetic resonance analysis of schizorhodopsin 1 in lipid bilayers."  Biophys. Chem. 335, 107635-107635 (2026).
PubMed: 42001687

Assembly members:

Assembly members:
entity_1, polymer, 201 residues, Formula weight is not available
entity_RET, non-polymer, 284.436 Da.

Natural source:

Natural source:   Common Name: Promethearchaeota archaeon   Taxonomy ID: 2053489   Superkingdom: not available   Kingdom: Promethearchaeati   Genus/species: Promethearchaeota Promethearchaeota archaeon

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEEIIFYIGAGVFTLTSITF FLLKKKNLEVASLNMIVNFV TIASYLLMVSGLFVASAVSG DSIYWTRWAFYAVSCSFLMV EISMLLSIDKSIKLEIIVFN CLVMITGLLASVSEGIIKWL FFTLSSVAYLYVLFQIIKHR SNEKFIVAFVAIFWSGFPII WILSPAGLMLIDAFWTALFY LVLDLITKVYFGYHTTLKFS K

Data sets:
Data typeCount
13C chemical shifts609
15N chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SzR11
2RETINAL2

Entities:

Entity 1, SzR1 201 residues - Formula weight is not available

1   METGLUGLUILEILEPHETYRILEGLYALA
2   GLYVALPHETHRLEUTHRSERILETHRPHE
3   PHELEULEULYSLYSLYSASNLEUGLUVAL
4   ALASERLEUASNMETILEVALASNPHEVAL
5   THRILEALASERTYRLEULEUMETVALSER
6   GLYLEUPHEVALALASERALAVALSERGLY
7   ASPSERILETYRTRPTHRARGTRPALAPHE
8   TYRALAVALSERCYSSERPHELEUMETVAL
9   GLUILESERMETLEULEUSERILEASPLYS
10   SERILELYSLEUGLUILEILEVALPHEASN
11   CYSLEUVALMETILETHRGLYLEULEUALA
12   SERVALSERGLUGLYILEILELYSTRPLEU
13   PHEPHETHRLEUSERSERVALALATYRLEU
14   TYRVALLEUPHEGLNILEILELYSHISARG
15   SERASNGLULYSPHEILEVALALAPHEVAL
16   ALAILEPHETRPSERGLYPHEPROILEILE
17   TRPILELEUSERPROALAGLYLEUMETLEU
18   ILEASPALAPHETRPTHRALALEUPHETYR
19   LEUVALLEUASPLEUILETHRLYSVALTYR
20   PHEGLYTYRHISTHRTHRLEULYSPHESER
21   LYS

Entity 2, RETINAL - C20 H28 O - 284.436 Da.

1   RET

Samples:

sample_1: SzR1, [U-100% 13C; U-100% 15N], 10 mg; ethanol 50%; H2O 50%

sample_conditions_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
DARRsample_1isotropicsample_conditions_1
NCACXsample_1isotropicsample_conditions_1
CONCAsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
2D 13C-detected NCAsample_1isotropicsample_conditions_1
1D 13Csample_1isotropicsample_conditions_1
1D 15Nsample_1isotropicsample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz