BMRB Entry 26317

Title:
Backbone 1H, 13C, 15N, HA and Side Chain 13C Chemical Shift Assignments for RRM3 of PABPC1
Deposition date:
2021-09-07
Original release date:
2022-05-11
Authors:
Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori
Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Sunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1"  J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
RRM3 of PABPC1, polymer, 104 residues, 11863 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p-1

Data sets:
Data typeCount
13C chemical shifts268
15N chemical shifts97
1H chemical shifts188

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 104 residues - 11863 Da.

1   GLYPROLEUGLYSERTHRASNVALTYRILE
2   LYSASNPHEGLYGLUASPMETASPASPGLU
3   ARGLEULYSASPLEUPHEGLYLYSPHEGLY
4   PROALALEUSERVALLYSVALMETTHRASP
5   GLUSERGLYLYSSERLYSGLYPHEGLYPHE
6   VALSERPHEGLUARGHISGLUASPALAGLN
7   LYSALAVALASPGLUMETASNGLYLYSGLU
8   LEUASNGLYLYSGLNILETYRVALGLYARG
9   ALAGLNLYSLYSVALGLUARGGLNTHRGLU
10   LEULYSARGLYSPHEGLUGLNMETLYSGLN
11   ASPARGILETHR

Samples:

sample_1: RRM3 of PABPC1, [U-100% 13C; U-100% 15N], 431 uM; sodium phosphate 18 mM; sodium chloride 135 mM; H2O 50 M; D2O, [U-2H], 10%; DTT 0.9 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CC(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks