BMRB Entry 26315

Title:
Backbone 1H, 15N and HA Chemical Shift Assignments for Paip2A(25-83)
Deposition date:
2021-09-07
Original release date:
2022-05-11
Authors:
Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori
Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1"  J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
Paip2A(25-83), polymer, 64 residues, 7995 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p-1

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts47
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 64 residues - 7995 Da.

1   GLYPROLEUGLYSERHISGLUASPASPASN
2   PROPHEALAGLUTYRMETTRPMETGLUASN
3   GLUGLUGLUPHEASNARGGLNILEGLUGLU
4   GLULEUTRPGLUGLUGLUPHEILEGLUARG
5   CYSPHEGLNGLUMETLEUGLUGLUGLUGLU
6   GLUHISGLUTRPPHEILEPROALAARGASP
7   LEUPROGLNTHR

Samples:

sample_1: Paip2A(25-83), [U-100% 15N], 90 uM; potassium phosphate 18 mM; sodium chloride 90 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks