BMRB Entry 26314

Name: Backbone 1H, 13C, 15N and Side chain CB Chemical Shift Assignments for Paip2A
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26314

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N and Side chain CB Chemical Shift Assignments for Paip2A

Deposition date:

2021-09-07

Original release date:

2022-05-11

Authors:

Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori

Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1" J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
Paip2A, polymer, 132 residues, 15396 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Paip2A: GPLGSMKDPSRSSTSPSIIN EDVIINGHSHEDDNPFAEYM WMENEEEFNRQIEEELWEEE FIERCFQEMLEEEEEHEWFI PARDLPQTMDQIQDQFNDLV ISDGSSLEDLVVKSNLNPNA KEFVPGVKYGNI

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	325
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 132 residues - 15396 Da.

1

GLY

PRO

LEU

GLY

SER

MET

LYS

ASP

PRO

SER

2

ARG

SER

THR

SER

PRO

SER

ILE

ASN

3

GLU

ASP

VAL

ILE

ASN

GLY

HIS

SER

HIS

4

GLU

ASP

ASN

PRO

PHE

ALA

GLU

TYR

MET

5

TRP

MET

GLU

ASN

GLU

PHE

ASN

ARG

6

GLN

ILE

GLU

LEU

TRP

GLU

7

PHE

ILE

GLU

ARG

CYS

PHE

GLN

GLU

MET

LEU

8

GLU

HIS

GLU

TRP

PHE

ILE

9

PRO

ALA

ARG

ASP

LEU

PRO

GLN

THR

MET

ASP

10

GLN

ILE

GLN

ASP

GLN

PHE

ASN

ASP

LEU

VAL

11

ILE

SER

ASP

GLY

SER

LEU

GLU

ASP

LEU

12

VAL

LYS

SER

ASN

LEU

ASN

PRO

ASN

ALA

13

LYS

GLU

PHE

VAL

PRO

GLY

VAL

LYS

TYR

GLY

14

ASN

ILE

Samples:

sample_1: Paip2A, [U-100% 13C; U-100% 15N], 218 uM; potassium phosphate 18 mM; sodium chloride 90 mM; H2O 90%; D2O, [U-100% 2H], 10%; DTT 0.9 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks