BMRB Entry 25905

Title:
Solution structure of the bromodomain of Trypanosoma brucei Bromodomain Factor 2(BDF2)
Deposition date:
2015-11-23
Original release date:
2016-11-17
Authors:
Yang, Xiao
Citation:

Citation: Yang, Xiao. "Solution structure of the bromodomain of Trypanosoma brucei Bromodomain Factor 2(BDF2)"  .

Assembly members:

Assembly members:
entity, polymer, 111 residues, 12723.368 Da.

Natural source:

Natural source:   Common Name: kinetoplastids   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Data sets:
Data typeCount
13C chemical shifts292
15N chemical shifts112
1H chemical shifts501

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 111 residues - 12723.368 Da.

1   METSERLYSASNGLUARGASPTHRSERPHE
2   ASNLYSASNGLYCYSLEUVALPHEVALSER
3   ARGLEUTRPASPLEUASPLYSLEUGLYMET
4   PHEHISHISPROVALSERALAGLUGLULEU
5   PROASPTYRHISTHRVALILELYSARGPRO
6   VALASPLEUSERSERILEARGASPGLYILE
7   GLULYSGLYTHRTYRALATHRASPVALASP
8   VALGLNASNASPVALALAARGMETILETHR
9   ASNALALEUGLUTYRASNALALYSGLYSER
10   THRTRPTYRGLNGLUALAMETSERPHEARG
11   LYSTHRTYRLEUASPLEUALAARGGLNSER
12   GLY

Samples:

sample_1: entity 0.5 mM; sodium chloride 150 mM; sodium phosphate 20 mM; DTT 1 mM; EDTA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DMX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks