BMRB Entry 25803

Title:
UBL domain of the yeast DNA damage-inducible protein homolog 1
Deposition date:
2015-09-09
Original release date:
2016-07-13
Authors:
Siva, Monika; Grantz Saskova, Klara; Veverka, Vaclav
Citation:

Citation: Klima, Martin; Toth, Daniel; Hexnerova, Rozalie; Baumlova, Adriana; Chalupska, Dominika; Tykvart, Jan; Rezabkova, Lenka; Sengupta, Nivedita; Man, Petr; Dubankova, Anna; Humpolickova, Jana; Nencka, Radim; Veverka, Vaclav; Balla, Tamas; Boura, Evzen. "Structural insights and in vitro reconstitution of membrane targeting and activation of human PI4KB by the ACBD3 protein"  Sci. Rep. 6, 23641-23641 (2016).
PubMed: 27009356

Assembly members:

Assembly members:
entity, polymer, 86 residues, 9692.008 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: n/a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts360
15N chemical shifts89
1H chemical shifts614

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 86 residues - 9692.008 Da.

1   HISILEGLUGLYARGHISMETASPLEUTHR
2   ILESERASNGLULEUTHRGLYGLUILETYR
3   GLYPROILEGLUVALSERGLUASPMETALA
4   LEUTHRASPLEUILEALALEULEUGLNALA
5   ASPCYSGLYPHEASPLYSTHRLYSHISASP
6   LEUTYRTYRASNMETASPILELEUASPSER
7   ASNARGTHRGLNSERLEULYSGLULEUGLY
8   LEULYSTHRASPASPLEULEULEUILEARG
9   GLYLYSILESERASNSER

Samples:

sample_1: sodium phosphate 25 mM; sodium chloride 100 mM; entity, [U-13C; U-15N], 0.4 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

YASARA, YASARA - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks