BMRB Entry 25735

Title:
The selective autophagy receptor TAX1BP1 is required for autophagy-dependent capture of cytosolic Salmonella typhimurium
Deposition date:
2015-07-29
Original release date:
2015-09-21
Authors:
Tumbarello, D; Manna, P; Allen, Mark; Bycroft, M; Kendrick-jones, J; Buss, F
Citation:

Citation: Tumbarello, David; Manna, Paul; Allen, Mark; Bycroft, Mark; Arden, Susan; Kendrick-Jones, John; Buss, Folma. "The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy"  PLoS Pathog. 11, e1005174-e1005174 (2015).
PubMed: 26451915

Assembly members:

Assembly members:
TAXBP1, polymer, 64 residues, 7679.4363 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI BL21(DE3)   Vector: HLTV

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts63
1H chemical shifts436

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TAXBP11
2ZINC ION, 12
3ZINC ION, 22

Entities:

Entity 1, TAXBP1 64 residues - 7679.4363 Da.

1   GLYSERSERPHEASPVALHISLYSLYSCYS
2   PROLEUCYSGLULEUMETPHEPROPROASN
3   TYRASPGLNSERLYSPHEGLUGLUHISVAL
4   GLUSERHISTRPLYSVALCYSPROMETCYS
5   SERGLUGLNPHEPROPROASPTYRASPGLN
6   GLNVALPHEGLUARGHISVALGLNTHRHIS
7   PHEASPGLNASN

Entity 2, ZINC ION, 1 - 65.409 Da.

1   ZN

Samples:

sample_1: TAXBP1, [U-13C; U-15N], 1.5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 100.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1
TOCSYsample_1solutionsample_conditions_1
DQF-COSYsample_1solutionsample_conditions_1
HSQCsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCACONHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HNCACOsample_1solutionsample_conditions_1
HNHBsample_1solutionsample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP TAXB1_HUMAN
AlphaFold Q9UI88

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks