BMRB Entry 25717

Title:
PltL-pyrrolyl
Deposition date:
2015-07-17
Original release date:
2015-09-16
Authors:
Jaremko, Matt; Lee, D.; Burkart, Michael
Citation:

Citation: Jaremko, Matt; Lee, D.; Opella, Stanley; Burkart, Michael. "Structure and Substrate Sequestration in the Pyoluteorin Type II Peptidyl Carrier Protein PltL"  J. Am. Chem. Soc. 137, 11546-11549 (2015).
PubMed: 26340431

Assembly members:

Assembly members:
entity, polymer, 90 residues, 10562.150 Da.
entity_4EM, non-polymer, 434.381 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 220664   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22b

Data sets:
Data typeCount
13C chemical shifts313
15N chemical shifts90
1H chemical shifts686

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1
2entity_4EM2

Entities:

Entity 1, entity 90 residues - 10562.150 Da.

1   METASPGLYGLUGLUVALLYSGLULYSILE
2   ARGARGTYRILEMETGLUASPLEUILEGLY
3   PROSERALALYSGLUASPGLULEUASPASP
4   GLNTHRPROLEULEUGLUTRPGLYILELEU
5   ASNSERMETASNILEVALLYSLEUMETVAL
6   TYRILEARGASPGLUMETGLYVALSERILE
7   PROSERTHRHISILETHRGLYLYSTYRPHE
8   LYSASPLEUASNALAILESERARGTHRVAL
9   GLUGLNLEULYSALAGLUSERALALEUGLU

Entity 2, entity_4EM - C16 H27 N4 O8 P - 434.381 Da.

1   4EM

Samples:

sample_1: entity, [U-13C; U-15N], mM; potassium phosphate 50 mM; TCEP 5 mM; sodium azide 0.1%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian VS 500 500 MHz
  • Bruker Avance 600 600 MHz
  • Varian VS 800 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks