BMRB Entry 25529

Name: NMR Solution Structure and Model Membrane Interaction Studies of the Peptide Hylin a1 from the Arboreal South American Frog Hypsiboas albopunctatus
Published: 2015-06-22

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PDB ID: 2n0o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25529
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Solution Structure and Model Membrane Interaction Studies of the Peptide Hylin a1 from the Arboreal South American Frog Hypsiboas albopunctatus

Deposition date:

2015-03-11

Original release date:

2015-06-22

Authors:

Alves, Eliane; Oliveira, Aline; Alves, Eliane

Citation:

Citation: Alves, Eliane; Junior, Edson; Cilli, Eduardo; Castro, Mariana; Fontes, Wagner; Magalhaes, Mariana; Liao, Luciano; Oliveira, Aline. "Micelle Bound Structure and Model Membrane Interaction Studies of the Peptide Hylin a1 from the Arboreal South American Frog Hypsiboas albopunctatus" Protein Pept. Lett. 22, 719-726 (2015).
PubMed: 26059694

Assembly members:

Assembly members:
entity, polymer, 18 residues, 1867.386 Da.

Natural source:

Natural source: Common Name: Spotted tree frog Taxonomy ID: 279985 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hypsiboas albopunctatus

Experimental source:

Experimental source: Production method: obtained from a collaborator Host organism: Hypsiboas albopunctatus

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: IFGAILPLALGALKNLIK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	123

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 18 residues - 1867.386 Da.

1

ILE

PHE

GLY

ALA

ILE

LEU

PRO

LEU

ALA

LEU

2

GLY

ALA

LEU

LYS

ASN

LEU

ILE

LYS

Samples:

sample_1: Hylin a1 1 mM; SDS-d25, [U-2H], 100 mM; D2O, [U-99% 2H], 10%; H2O 90%; TMSP 1 mg/uL

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR_NIH v2.28, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

ProcheckNMR, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Thornton - data analysis

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

Bruker Avance 500 MHz