BMRB Entry 25339

Title:
Backbone amide assignments for human Cyclophilin C with saturating concentrations of the model peptide GSFGPDLRAGD
Deposition date:
2014-11-13
Original release date:
2014-12-16
Authors:
Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan
Citation:

Citation: Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan. "Structure and Dynamics of GeoCyp: A Thermophilic Cyclophilin with a Novel Substrate Binding Mechanism That Functions Efficiently at Low Temperatures"  Biochemistry 54, 3207-3217 (2015).
PubMed: 25923019

Assembly members:

Assembly members:
CypC, polymer, 190 residues, 20515.3 Da.
GSFGPDLRAGD, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28

Data sets:
Data typeCount
15N chemical shifts175
1H chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CypC1
2GSFGPDLRAGD2

Entities:

Entity 1, CypC 190 residues - 20515.3 Da.

Residues 25-212 of human Cyclophilin C with two additional N-terminal residues, Gly-Ser-, remaining after removal of affinity tag

1   GLYSERGLYALAGLUGLYPHEARGLYSARG
2   GLYPROSERVALTHRALALYSVALPHEPHE
3   ASPVALARGILEGLYASPLYSASPVALGLY
4   ARGILEVALILEGLYLEUPHEGLYLYSVAL
5   VALPROLYSTHRVALGLUASNPHEVALALA
6   LEUALATHRGLYGLULYSGLYTYRGLYTYR
7   LYSGLYSERLYSPHEHISARGVALILELYS
8   ASPPHEMETILEGLNGLYGLYASPILETHR
9   THRGLYASPGLYTHRGLYGLYVALSERILE
10   TYRGLYGLUTHRPHEPROASPGLUASNPHE
11   LYSLEULYSHISTYRGLYILEGLYTRPVAL
12   SERMETALAASNALAGLYPROASPTHRASN
13   GLYSERGLNPHEPHEILETHRLEUTHRLYS
14   PROTHRTRPLEUASPGLYLYSHISVALVAL
15   PHEGLYLYSVALILEASPGLYMETTHRVAL
16   VALHISSERILEGLULEUGLNALATHRASP
17   GLYHISASPARGPROLEUTHRASNCYSSER
18   ILEILEASNSERGLYLYSILEASPVALLYS
19   THRPROPHEVALVALGLUILEALAASPTRP

Entity 2, GSFGPDLRAGD 11 residues - Formula weight is not available

1   GLYSERPHEGLYPROASPLEUARGALAGLY
2   ASP

Samples:

CypC-GSFGPDLRAGD: CypC, [U-13C; U-15N; U-2H], 1 mM; GSFGPDLRAGD 6 mM; sodium phosphate 50 mM; EDTA 1 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCCypC-GSFGPDLRAGDisotropicsample_conditions
3D 1H-15N NOESYCypC-GSFGPDLRAGDisotropicsample_conditions

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, peak picking

nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Varian - Spectrum processing, collection

NMR spectrometers:

  • Varian UnityInova 900 MHz

Related Database Links:

BMRB 25341
PDB
DBJ BAF83010
GB AAB31350 AAH02678 ABO43038 ADQ32383 EAW48878
REF NP_000934 NP_001180902 XP_002815869 XP_003310839 XP_003826742
SP P45877
AlphaFold P45877

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks