BMRB Entry 25335

Title:
Backbone and 1H assignments for VG16KRKP, an antimicrobial peptide in LPS
Deposition date:
2014-11-13
Original release date:
2014-12-22
Authors:
Bhunia, Anirban; Datta, Aritreyee
Citation:

Citation: Bhunia, Anirban; Datta, Aritreyee. "denovo design of antimicrobial peptide:insights into outer membrane permeabilisation, LPs fragmentation and application in treating plant disease"  .

Assembly members:

Assembly members:
entity, polymer, 17 residues, 1765.171 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: VARGWKRKCPLFGKGGG

Data sets:
Data typeCount
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 17 residues - 1765.171 Da.

1   VALALAARGGLYTRPLYSARGLYSCYSPRO
2   LEUPHEGLYLYSGLYGLYGLY

Samples:

sample_1: H2O 90%; D2O 10%; TSP 1 mM; entity 1 mM

sample_conditions_1: temperature: 298 K; pH: 4.5; pressure: 1 atm; ionic strength: 0 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 25408 25409
PDB