BMRB Entry 25325

Title:
NMR Chemical Shifts of the hybrid protein Beta-Synuclien HC
Deposition date:
2014-11-10
Original release date:
2015-09-03
Authors:
Allison, Jane; Rivers, Robert; Christodoulou, John; Vendruscolo, Michele; Dobson, Christopher
Citation:

Citation: Allison, Jane; Rivers, Robert; Christodoulou, John; Vendruscolo, Michele; Dobson, Christopher. "A relationship between the transient structure in the monomeric state and the aggregation propensities"  Biochemistry ., .-..

Assembly members:

Assembly members:
beta-synuclein_HC, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: T9

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts133
1H chemical shifts133

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Beta-synuclein HC1

Entities:

Entity 1, Beta-synuclein HC 145 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERMET
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALTHRGLUALAALA
4   GLULYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRARGGLUGLYVALVALGLN
6   GLYVALALASERVALALAGLULYSTHRLYS
7   GLUGLNALASERHISLEUGLYGLYALAVAL
8   PHESERGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYASNILEALAALA
10   ALATHRGLYLEUVALLYSARGGLUGLUPHE
11   PROTHRASPLEULYSPROGLUGLUVALALA
12   GLNGLUALAALAGLUGLUPROLEUILEGLU
13   PROLEUMETGLUPROGLUGLYGLUSERTYR
14   GLUASPPROPROGLNGLUGLUTYRGLNGLU
15   TYRGLUPROGLUALA

Samples:

sample: beta-synuclein HC, [U-99% 13C; U-99% 15N], 150 uM; NaCl 100 mM; Phosphate Buffer 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsampleisotropicsample_conditions_1
3D HNCOsampleisotropicsample_conditions_1
2D 1H-15N HSQCsampleisotropicsample_conditions_1

Software:

SPARKY v3.114, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks