BMRB Entry 25306

Title:
1H, 13C and 15N chemical shift assignments for Oscillatoria agardhii agglutinin
Deposition date:
2014-10-30
Original release date:
2015-08-25
Authors:
Lee, Donghan; Carneiro, Marta; Koharudin, Leonardus; Griesinger, Christian; Gronenborn, Angela
Citation:

Citation: Carneiro, Marta; Koharudin, Leonardus; Griesinger, Christian; Gronenborn, Angela; Lee, Donghan. "1H, 13C and 15N resonance assignment of the anti-HIV lectin from Oscillatoria agardhii"  Biomol. NMR Assign. 9, 317-319 (2015).
PubMed: 25680849

Assembly members:

Assembly members:
OAA, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Planktothrix agardhii   Taxonomy ID: 1160   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Planktothrix agardhii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET26B

Data sets:
Data typeCount
13C chemical shifts511
15N chemical shifts157
1H chemical shifts839

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OAA monomer1

Entities:

Entity 1, OAA monomer 133 residues - Formula weight is not available

1   METALALEUTYRASNVALGLUASNGLNTRP
2   GLYGLYSERSERALAPROTRPASNGLUGLY
3   GLYGLNTRPGLUILEGLYSERARGSERASP
4   GLNASNVALVALALAILEASNVALGLUSER
5   GLYASPASPGLYGLNTHRLEUASNGLYTHR
6   METTHRTYRALAGLYGLUGLYPROILEGLY
7   PHEARGALATHRLEULEUGLYASNASNSER
8   TYRGLUVALGLUASNGLNTRPGLYGLYASP
9   SERALAPROTRPHISSERGLYGLYASNTRP
10   ILELEUGLYSERARGGLUASNGLNASNVAL
11   VALALAILEASNVALGLUSERGLYASPASP
12   GLYGLNTHRLEUASNGLYTHRMETTHRTYR
13   ALAGLYGLUGLYPROILEGLYPHELYSGLY
14   THRLEUTHR

Samples:

15N: OAA, [U-100% 15N], 2 mM; sodium chloride 20 mM; sodium acetate 20 mM; sodium azide 3 mM

13C15N: OAA, [U-100% 13C; U-100% 15N], 2 mM; sodium chloride 20 mM; sodium acetate 20 mM; sodium azide 3 mM

13C15N_d2o: OAA, [U-100% 13C; U-100% 15N], 2 mM; sodium chloride 20 mM; sodium acetate 20 mM; sodium azide 3 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15Nisotropicsample_conditions_1
2D 1H-13C HSQC aliphatic13C15N_d2oisotropicsample_conditions_1
2D 1H-13C HSQC aromatic13C15N_d2oisotropicsample_conditions_1
3D HNCA13C15Nisotropicsample_conditions_1
3D HNCO13C15Nisotropicsample_conditions_1
3D HCCH-TOCSY13C15N_d2oisotropicsample_conditions_1
3D 1H-15N NOESY15Nisotropicsample_conditions_1
3D 1H-13C NOESY aliphatic13C15Nisotropicsample_conditions_1
3D 1H-13C NOESY aromatic13C15Nisotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks