BMRB Entry 25265

Title:
Structural insight into an essential assembly factor network on the pre-ribosome
Deposition date:
2014-10-02
Original release date:
2014-12-01
Authors:
Lee, Woonghee; BaBler, Jochen; Paternoga, Helge; Holdermann, Iris; Thomas, Matthias; Granneman, Sander; Barrio-Garcia, Clara; Nyarko, Afua; Stier, Gunter; Clark, Sarah; Schraivogel, Daniel; Kallas, Martina; Beckmann, Roland; Tollervey, David; Barbar, Elisar; Sinning, Irmi; Hurt, Ed
Citation:

Citation: Lee, Woonghee; BaBler, Jochen; Paternoga, Helge; Holdermann, Iris; Thomas, Matthias; Granneman, Sander; Barrio-Garcia, Clara; Nyarko, Afua; Stier, Gunter; Clark, Sarah; Schraivogel, Daniel; Kallas, Martina; Beckmann, Roland; Tollervey, David; Barbar, Elisar; Sinning, Irmi; Hurt, Ed. "A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation"  J. Cell Biol. 207, 481-498 (2014).
PubMed: 25404745

Assembly members:

Assembly members:
entity, polymer, 188 residues, 20618.100 Da.

Natural source:

Natural source:   Common Name: ascomycetes   Taxonomy ID: 209285   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Chaetomium thermophilum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts365
15N chemical shifts86
1H chemical shifts636

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 188 residues - 20618.100 Da.

1   TYRGLUARGPHEILEARGPROMETGLYLEU
2   ARGTYRLYSLYSALAASNVALTHRHISPRO
3   THRLEUASNVALTHRVALGLNLEUPROILE
4   LEUSERVALLYSLYSASNPROSERASNPRO
5   LEUTYRTHRGLNLEUGLYVALLEUTHRLYS
6   GLYTHRILEILEGLUVALASNVALSERASP
7   LEUGLYILEVALTHRALASERGLYLYSILE
8   ALATRPGLYARGTYRALAGLNILETHRASN
9   ASNPROGLUASNASPGLYCYSVALASNALA
10   VALLEULEUVALTYRGLUARGPHEILEARG
11   PROMETGLYLEUARGTYRLYSLYSALAASN
12   VALTHRHISPROTHRLEUASNVALTHRVAL
13   GLNLEUPROILELEUSERVALLYSLYSASN
14   PROSERASNPROLEUTYRTHRGLNLEUGLY
15   VALLEUTHRLYSGLYTHRILEILEGLUVAL
16   ASNVALSERASPLEUGLYILEVALTHRALA
17   SERGLYLYSILEALATRPGLYARGTYRALA
18   GLNILETHRASNASNPROGLUASNASPGLY
19   CYSVALASNALAVALLEULEUVAL

Samples:

sample_1: D2O 10%; TRIS 10 mM; H2O 90%; sodium chloride 5 mM; DSS 1 uM; TCEP 2 mM; entity, [U-99% 13C; U-99% 15N], 450 mM

sample_conditions_1: ionic strength: 5 mM; pH: 7.4; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich, PONDEROSA-C/S and NMRFAM-SPARKY - peak picking, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz
  • Bruker Avance 950 MHz

Related Database Links:

PDB
GB AEO54371 AEO65388 EGS23242
REF XP_003651724 XP_003659616 XP_006691433

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks