BMRB Entry 25181

Title:
Three-Dimensional Structure and Interaction Studies of Hepatitis C Virus p7 in 1,2-Dihexanoyl-sn-glycero-3-phosphocholine by Solution Nuclear Magnetic Resonance
Deposition date:
2014-08-29
Original release date:
2014-10-07
Authors:
Cook, Gabriel; Dawson, Lindsay; Tian, Ye; Opella, Stanley
Citation:

Citation: Cook, Gabriel; Dawson, Lindsay; Tian, Ye; Opella, Stanley. "Three-dimensional structure and interaction studies of hepatitis C virus p7 in 1,2-dihexanoyl-sn-glycero-3-phosphocholine by solution nuclear magnetic resonance."  Biochemistry 52, 5295-5303 (2013).
PubMed: 23841474

Assembly members:

Assembly members:
entity, polymer, 63 residues, 6693.997 Da.

Natural source:

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 420174   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHLV

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts61
1H chemical shifts58
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 63 residues - 6693.997 Da.

1   ALALEUGLUASNLEUVALVALLEUASNALA
2   ALASERVALALAGLYALAHISGLYILELEU
3   SERPHELEUVALPHEPHESERALAALATRP
4   TYRILELYSGLYARGLEUALAPROGLYALA
5   ALATYRALAPHETYRGLYVALTRPPROLEU
6   LEULEULEULEULEUALALEUPROPROARG
7   ALATYRALA

Samples:

sample_1: p7, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; DHPC 125 mM; D2O 10%

sample_2: p7, [U-100% 15N], 0.5 mM; DHPC 125 mM; D2O 10%; H2O 90%; polyacrylamide gel 6%

sample_conditions_1: temperature: 323 K; pH: 4.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N HSQC NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 18863 25178
PDB
DBJ BAA01583 BAA02756
EMBL CAA43792
GB AAC15722 AAC15723 AAC15724 AAC15725 AAC15726
SP O92972
AlphaFold O92972

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks