BMRB Entry 25161

Title:
Carnolysin A1'
Deposition date:
2014-08-18
Original release date:
2014-09-25
Authors:
Lohans, Christopher; Li, Jessica; Vederas, John
Citation:

Citation: Lohans, Christopher; Li, Jessica; Vederas, John. "Structure and Biosynthesis of Carnolysin, a Homolog of Enterococcal Cytolysin with D-Amino Acids"  J. Am. Chem. Soc. 136, 13150-13153 (2014).
PubMed: 25207953

Assembly members:

Assembly members:
CrnA1', polymer, 45 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 2751   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Carnobacterium maltaromaticum

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Carnobacterium maltaromaticum   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CrnA1': GDINGEFXXXPACVYXVMVV XKAAXAKCAAGAXAVXGAIL XAIRC

Data sets:
Data typeCount
1H chemical shifts225

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Carnolysin A1'1

Entities:

Entity 1, Carnolysin A1' 45 residues - Formula weight is not available

1   GLYASPILEASNGLYGLUPHEDBUABADHA
2   PROALACYSVALTYRDALVALMETVALVAL
3   DALLYSALAALADHAALALYSCYSALAALA
4   GLYALADALALAVALDALGLYALAILELEU
5   DALALAILEARGCYS

Samples:

sample_1: CrnA1' 1 mM; DSS 0.01%; H2O 90%; D2O 10%

sample_conditions_1: temperature: 273 K; pH: 6; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian VXRS 700 MHz