BMRB Entry 21100

Title:
Solution structure of M-MYRTX-Tb1a peptide (Bicarinalin) from Ant peptide venom.
Deposition date:
2022-07-12
Original release date:
2022-12-24
Authors:
PAQUET, Francoise; JOUVENSAL, Laurence; LOTH, Karine
Citation:

Citation: Ascoet, Steven; Touchard, Axel; Tene, Nathan; Lefranc, Benjamin; Leprince, Jerome; Paquet, Francoise; Jouvensal, Laurence; Barasse, Valentine; Treilhou, Michel; Billet, Arnaud; Bonnafe, Elsa. "The mechanism underlying toxicity of a venom peptide against insects reveals how ants are master at disrupting membranes."  iScience 26, 106157-106157 (2023).
PubMed: 36879819

Assembly members:

Assembly members:
M-MYRTX-Tb1a, polymer, 20 residues, 2235.839 Da.

Natural source:

Natural source:   Common Name: ants   Taxonomy ID: 219812   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tetramorium bicarinatum

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M-MYRTX-Tb1a: KIKIPWGKVKDFLVGGMKAV X

Data sets:
Data typeCount
13C chemical shifts80
15N chemical shifts18
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1M-MYRTX-Tb1a1

Entities:

Entity 1, M-MYRTX-Tb1a 20 residues - 2235.839 Da.

1   LYSILELYSILEPROTRPGLYLYSVALLYS
2   ASPPHELEUVALGLYGLYMETLYSALAVAL
3   NH2

Samples:

sample_1: M-MYRTX-Tb1a 1.2 ± 0.05 mM; TFE-d2, [U-2H], 100%

sample_conditions_1: pH: 4.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

ARIA v2.3.1, Linge, O'Donoghue and Nilges - structure solution

CCPNMR v2.1, CCPN - chemical shift assignment, data analysis, peak picking

TOPSPIN v3.6.2, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance III HD 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks